Phosphorylation of Wild Soybean (Glycine soja) Splicing Factor GsSCL30a by GsSnRK1 Regulates Soybean Tolerance to Alkali Stress
文献类型： 外文期刊
作者： Li, Minglong 1 ; Liu, Xin 1 ; Zhao, Xin 1 ; Liu, Fujing 1 ; Xue, Man 1 ; Liu, Haishan 1 ; Sun, Xiaohuan 3 ; Liu, Changyuan 3 ; Liu, Zhangxiong 4 ; Ding, Xiaodong 1 ; Li, Qiang 1 ; Xiao, Jialei 1 ;
作者机构： 1.Northeast Agr Univ, Key Lab Agr Biol Funct Genes, Harbin, Peoples R China
2.Northeast Agr Univ, Key Lab Soybean Biol Chinese Educ Minist, Harbin, Peoples R China
3.Heilongjiang Acad Agr Sci, Mudanjiang Branch, Mudanjiang, Peoples R China
4.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improve, Key Lab Soybean Biol Beijing MOA,Key Lab Crop Gene, Beijing, Peoples R China
关键词： alkaline stress; cultivated soybean; GsSCL30a; splicing factor; wild soybean
期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：6.3； 五年影响因子：7.7 ）
ISSN： 0140-7791
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Pre-mRNA splicing is a fundamental process in plant gene expression, and the resulting transcripts may play significant roles in plant development and responses to environmental changes. Wild soybean (Glycine soja), as a close relative of cultivated soybean (Glycine max), has excellent traits such as alkali tolerance and is an ideal material for mining stress-resistant genes. Previous phosphoproteomic analysis identified SR protein GsSCL30a as a potential phosphorylation substrate of GsSnRK1 kinase. Y2H and BiFC assays confirmed their interaction. In vitro phosphorylation and mass spectrometry showed GsSnRK1 phosphorylates six residues in GsSCL30a. RT-qPCR revealed GsSCL30a is ubiquitously expressed, with alkali stress regulating its transcript levels. Alternative splicing analysis showed GsSCL30a acts as a splicing factor, self-splicing its third intron with conserved GAAG motifs and GsSnRK1 phosphorylation enhances this activity. GsSCL30a interacts with U1-70K, suggesting involvement in U1-RNP complex at 5 ' splice sites. Soybean hairy root overexpression showed GsSCL30a-GsSnRK1 synergistically enhances alkali tolerance. This study revealed the molecular mechanism of GsSnRK1-GsSCL30a module in response to alkali stress and provides a new idea to breed the high-resistant crops.
- 相关文献
作者其他论文 更多>>
-
A comparison of Chinese soybean collection with European soybean collections on genetic diversity by genome-wide scan
作者：Xu, Jiangyuan;Lu, Yuqing;Gu, Yongzhe;Gao, Huawei;Liu, Zhangxiong;Qiu, Lijuan;Yao, Xindong;Yao, Xindong;Leopold, Rittler;Yuan, Ming;Zhang, Yong;Sun, Rujian;Xue, Yongguo;Liu, Yeli;Han, Dezhi;Wang, Jinxing;Qiu, Lijuan
关键词：Chinese soybean; European soybean; Genetic diversity; Selective sweep analysis; Selected region
-
A wheat phytohormone atlas spanning major tissues across the entire life cycle provides novel insights into cytokinin and jasmonic acid interplay
作者：Yin, Huanran;Liu, Wei;Hu, Xin;Jia, Jingqi;Liu, Mengmeng;Wei, Jiaqi;Cheng, Yikeng;Gong, Xin;Li, Qiang;Yan, Wenhao;Chen, Wei;Jia, Jizeng;Gao, Lifeng;Fernie, Alisdair R.
关键词：wheat; phytohormones; metabolic regulatory network; DOF
-
Knockout of phosphatidate phosphohydrolase genes confers broad-spectrum disease resistance in plants
作者：Gong, Qiuwen;Sha, Gan;Han, Xinyu;Guo, Zhenhua;Yang, Lei;Yang, Wei;Tan, Ronglei;Liu, Meng;Xia, Fengdie;Li, Guotian;Sha, Gan;Chen, Ting;Chen, Guang;Shen, Xin;Xie, Kabin;Hu, Honghong;Li, Qiang;Li, Yufei;Luo, Jie;Cai, Guangqin;Luo, Jie
关键词：genome-editing; phosphatidic acid; Oryza sativa; Arabidopsis; Magnaporthe oryzae
-
Optimization of Irrigation Parameters of Peanut Under Mulched Drip Irrigation in Xinjiang Based on Yield and Water Use Efficiency
作者：Zhang, Yuchao;Li, Shaofei;Li, Haiming;Chen, Junwei;Dong, Jianshu;Shen, Xiaojun;Zhang, Yuchao;Gao, Yang;Si, Zhuanyun;Li, Haiming;Chen, Junwei;Dong, Jianshu;Shen, Xiaojun;Cui, Weimin;Li, Qiang;Zhang, Xiaopei
关键词：drip discharge; peanut; yield; quality; water use efficiency; nitrogen partial factor productivity
-
Molecular mechanism of potently neutralizing human monoclonal antibodies against severe fever with thrombocytopenia virus infection
作者：Quan, Chuansong;Nie, Kaixiao;Zheng, Wenjun;Wang, Yiwen;Quan, Chuansong;Zhang, Hong;Quan, Chuansong;Ma, Dezhen;Su, Chao;Li, Lianfeng;Peng, Dingkun;Yin, Chunhong;Yang, Peipei;Shan, Chao;Liu, Xin;Zheng, Jie;Shi, Weifeng;Li, Weiwei;Gao, George F.;Qi, Jianxun;Liu, Weixiao;Liu, Di;Carr, Michael J.;Carr, Michael J.;Shi, Weifeng
关键词：severe fever with thrombocytopenia syndrome; SFTSV; Bandavirus dabieense; monoclonal antibodies; neutralization; therapeutics
-
Enhancing the synthesis efficiency of galacto-oligosaccharides of a β-galactosidase from Paenibacillus barengoltzii by engineering the active and distal sites
作者：Yu, Haiyan;Wang, Yulu;Ying, Jiabao;Liu, Bolin;Mohamed, Abeer;Wei, Xue;Yang, Yuji;Xin, Fengjiao;Yang, Zhisen;Liu, Xin;Sun, Linfeng;Guan, Feifei;Miao, Miao;Jiang, Zhengqiang;Yang, Shaoqing;Wang, Yulu;Xin, Fengjiao
关键词：beta-Galactosidase; GOS synthesis; Cryo-EM structure; Machine learning; Catalytic mechanism
-
Sustainable Weed Management: The Effects of Applying Pre- and Post-Emergence Herbicides to Medicago ruthenica
作者：Li, Qiang;Ren, Zhongwei;Xu, Hui;Wang, Wenying;Yu, Linqing;Li, Jun;Li, Qiang;Zhang, Yarong;Huang, Fan
关键词：weed management; herbicides;
Medicago ruthenica ; sustainable agriculture
