您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Butylated hydroxyanisole controls anthracnose on postharvest citrus fruit by inducing autophagosome formation in Colletotrichum gloeosporioides

文献类型： 外文期刊

作者： Wang, Yuqing 1 ; Ji, Sirong 1 ; Chen, Xiao 1 ; Liu, Shuqi 1 ; Zhao, Juan 1 ; Long, Chao-an 1 ;

作者机构： 1.Huazhong Agr Univ, Natl R&D Ctr Citrus Preservat, Natl Ctr Citrus Breeding, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Wuhan 430070, Peoples R China

3.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch,Minist Agr,Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr,Genome Anal Lab, Shenzhen 518000, Peoples R China

关键词： Citrus; Anthracnose; Butylated hydroxyanisole; Autophagy; ROS; Membrane integrity

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：7.0； 五年影响因子：6.9 ）

ISSN： 0925-5214

年卷期： 2024 年 210 卷

页码：

收录情况： SCI

摘要： Citrus anthracnose, caused by Colletotrichum gloeosporioides, is a preharvest and postharvest disease that results in economic losses by affecting citrus fruit quality, shelf life, and market value. To date, research on environmental safety, high efficiency and viable alternatives has not been reported. In this study, butylated hydroxyanisole (BHA) was screened and verified to inhibit C. gloeosporioides of citrus. The results showed that BHA could completely inhibit mycelial growth in vitro (0.25 g/L) and absolutely lose its virulence in vivo (20 x Minimum fungicidal concentration (MFC) = 5 g/L). Furthermore, some transparent irregularly shaped vacuolar structures in the cytosol of the BHA-treated conidia were detected by Transmission Electron Microscope (TEM), and autophagosome formation was verified by monodansylcadaverine(MDC) staining. In addition, staining experiments indicated that BHA induced the accumulation of excessive reactive oxygen species (ROS, the DCFH-DA staining rates of C. gloeosporioides conidia treated with 1/2 x MIC and MIC were 26.4% and 83%, respectively), which implied that BHA destroyed spore organelles and damaged cell membrane integrity (the electrical conductivity of conidia treated with 1/2 x Minimum inhibitory concentration (MIC), MIC and MFC was 48.37%, 64.79% and 97.95%, respectively), further resulting in reduced pathogenicity and virulence. In summary, this study provides evidence that BHA has the potential to be a valuable alternative for controlling citrus anthracnose in the future.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台