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Functional analysis of potential fumiquinazoline biosynthetic gene clusters in Penicillium digitatum of citrus

文献类型： 外文期刊

作者： Liu, Shuqi 1 ; Lu, Yongqing 1 ; Wang, Yuqing 1 ; Fang, Wei 4 ; Long, Chao-an 1 ;

作者机构： 1.Huazhong Agr Univ, Natl R&D Ctr Citrus Preservat, Natl Ctr Citrus Breeding, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Wuhan 430070, Peoples R China

3.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr,Genome Anal Lab,M, Shenzhen 518000, Peoples R China

4.Hubei Acad Agr Sci, Hubei Biopesticide Engn Res Ctr, 8 Nanhu Ave, Wuhan 430064, Peoples R China

关键词： Penicillium digitatum; Secondary metabolites; Gene; Virulence

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：6.8； 五年影响因子：7.5 ）

ISSN： 0925-5214

年卷期： 2025 年 222 卷

页码：

收录情况： SCI

摘要： Penicillium digitatum is a pathogenic fungus responsible for the postharvest green mold of citrus, leading to significant economic losses in the citrus industry. Filamentous fungi have a rich secondary metabolic network, and their products have important applications in medicine. Several secondary metabolites (tryptoquialanines, fumiquinazolines, and so on) have been identified in P. digitatum. However, the molecular networks regulating the biosynthesis of secondary metabolites and their gene functions remain to be investigated. For functional analysis of the genes, a homologous recombination knockout strategy was used to generate knockout mutants of seven genes putative to be involved in the fumiquinazoline (FQ) biosynthesis pathway in Penicillum digitatum. LC- MS analysis revealed that the FQ biosynthetic gene clusters mainly regulated tryptoquialanine biosynthesis in P. digitatum. Virulence assessment experiments with knockout mutants displayed that compared with that in the wild type, the onset of green mold was delayed in all the citrus groups inoculated with the seven mutants, revealing that the involvement of the FQ biosynthetic gene clusters positively regulates the virulence of P. digitatum. Furthermore, the survival of the knockout mutants was greatly decreased under ultraviolet (UV-C) radiation, indicating that this gene cluster participated in the UV-C sensitivity of P. digitatum. The present study also revealed that genes in FQ biosynthetic gene clusters regulate low-temperature stress resistance and KCl stress resistance. In summary, this study determined that the FQ biosynthetic gene clusters regulate the biosynthesis of SMs and respond to multiple biological processes in P. digitatum.

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