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A Visual Gel-Enzyme-Linked Immunosorbent Assay for Simultaneous Detection of Gentamicin and Kanamycin in Milk

文献类型: 外文期刊

作者: Xu Fei 1 ; Li Jing-Ya 2 ; Zhou Jie 2 ; Liu Mao-Lin 1 ; Liu Yi-Ming 1 ; Wang Jian-Fen 3 ; Ding Shuang-Yang 2 ; Li Xiu-Bo 1 ;

作者机构: 1.Chinese Acad Agr Sci, Feed Res Inst, Natl Feed Drug Reference Labs, Beijing 100081, Peoples R China

2.China Agr Univ, Natl Reference Lab Residues Vet Drugs, Beijing 100193, Peoples R China

3.Yanqing Cty Anmial Husb Tech Advice Stn Beijing, Beijing 102100, Peoples R China

关键词: Gel-enzyme-linked immunosorbent assay;Gentamicin;Kanamycin;Simultaneous detection;Milk

期刊名称:CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ( 影响因子:1.134; 五年影响因子:0.909 )

ISSN: 0253-3820

年卷期: 2015 年 43 卷 6 期

页码:

收录情况: SCI

摘要: A visual gel-enzyme-linked immunosorbent assay (Gel-ELISA) assay for simultaneous detection of gentamicin (GEN) and kanamycin (KANA) in milk was described. In the gel detection column, two detection layers were filled with CN-Br activated Sephrose 4B gel as solid phase carrier, which was coupled with goat anti-mouse IgG. The anti-GEN and anti-KANA monoclonal antibodies were added to combine with the carrier respectively in each layer. Gentamicin and kanamycin drugs in sample competed with corresponding enzyme-labeled antigens for the immobilized antibody on the column, and the drugs could be detected by the chromogenic reaction of substrate 3,3' ,5, 5' -tetramethylbenzidine. This approach was a yes/no method after color detection by naked eyes with the detection time of 15 min. The sensitivity of the Gel-ELISA method was 2 mu g/L, and the detection limit (Cut-off value) for gentamicin and kanamycin in raw milk was 5 mu g/L. The detection results of the ten blind milk samples by the proposed Gel-ELISA method were consistent with those obtained by ultra performance liquid chromatography. tandem mass spectrometry (UPLC/MS-MS).

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