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K-Domain Splicing Factor OsMADS1 Regulates Open Hull Male Sterility in Rice

文献类型： 外文期刊

作者： Sun Lian-ping 1 ; Zhang Ying-xin 1 ; Zhang Pei-pei 1 ; Yang Zheng-fu 1 ; Zhan Xiao-deng 1 ; Shen Xi-hong 1 ; Zhang Zhe 1 ;

作者机构： 1.China Natl Rice Res Inst, Key Lab Zhejiang Super Rice Res, Natl Ctr Rice Improvement, Hangzhou 310006, Zhejiang, Peoples R China

2.Shenyang Agr Univ, Coll Agron, Shenyang 110866, Peoples R China

关键词： rice;open hull;male sterile;ohms1;gene mapping;alternative splicing;floral organ

期刊名称：RICE SCIENCE （ 影响因子：3.333； 五年影响因子：4.226 ）

ISSN： 1672-6308

年卷期： 2015 年 22 卷 5 期

页码：

收录情况： SCI

摘要： We identified the rice floral organ development mutant, termed as open hull and male sterile 1 (ohms1), from the progeny of the indica restorer line Zhonghui 8015 treated with Co-60 Y-ray irradiation. The ohms1 mutant exhibited an open hull and lemma-and palea-like structure conversion between the anthers and stigma, which resulted in the ohms1 mutant spikelet showing 'tridentate lemma'. The ohms1 mutant was entirely sterile but had 60%-70% fertile pollen. Genetic analysis and gene mapping showed that ohms1 was controlled by a single recessive gene, and the mutant gene was fine-mapped to a 42-kb interval on the short arm of chromosome 3 between markers KY2 and KY29. Sequence analysis of the four open reading frames in this region revealed that the mutant carried a single nucleotide transformation (A to G) at the last base of the fifth intron, which was likely corresponded to ohms1 phynotype, in an MIKC type MADS-box gene OsMADS1 (LOC_ Os03g11614). Enzyme digestion and cDNA sequencing further indicated that the variable splicing was responsible for the deletion of the sixth exon in ohms1, but no structural changes in the MADS domain or amino acid frame shifts appeared. Additionally, real-time fluorescent quantitative PCR analysis showed that the OsMADS1 expression level decreased significantly in the ohms1 mutant. The expression levels of rice flowering factors and floral glume development-related genes also changed significantly. These results demonstrate that OsMADS1 may play an important role in rice floral organ development, particularly in floral glume development and floret primordium differentiation.

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