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Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3

文献类型: 外文期刊

作者: Liu, X. 1 ; Wang, L. G. 1 ; Zhang, L. C. 1 ; Yan, H. 1 ; Zhao, K. B. 1 ; Liang, J. 1 ; Li, N. 1 ; Pu, L. 2 ; Zhang, T.; Wang 1 ;

作者机构: 1.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr China, Key Lab Farm Anim Genet Resources & Germplasm Inn, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr Chi

关键词: Cloning;Copy number variation;mRNA expression;Porcine;Signal peptide CUB EGF-like domain-containing protein 3 gene

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2016 年 15 卷 1 期

页码:

收录情况: SCI

摘要: The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The full-length sequence of porcine SCUBE3 cDNA was 4131 base pairs and included 21 exons. The SCUBE3 gene contained a 2895-base pair open reading frame that encoded a peptide of 965 amino acids. Comparison of the deduced amino acid sequences of porcine SCUBE3 with those of human, mouse, zebrafish, and rat showed 96, 95, 73, and 95% identities, respectively. Porcine SCUBE3 mRNA expression levels were highest in the backfat, bone marrow, and cartilage tissues. Copy number variation was detected in porcine SCUBE3 and validated by real-time quantitative polymerase chain reaction. Different copy number variations were present in randomly selected individuals and may, therefore, be a good marker for identifying phenotypic traits. Our findings provide a basis for further investigation of the functions and regulatory mechanisms of SCUBE3 in pigs.

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