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Co-expression of AtNHX1 and TsVP improves the salt tolerance of transgenic cotton and increases seed cotton yield in a saline field

文献类型： 外文期刊

作者： Cheng, Cheng 1 ; Zhang, Ying 1 ; Chen, Xiugui 1 ; Song, Jiuling 1 ; Guo, Zhiqiang 1 ; Li, Kunpeng 1 ; Zhang, Kewei 2 ;

作者机构： 1.School of Life Science, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China;Ministry of Education Key Laboratory of Plant Cell Engineering and Germplasm Enhancement, Jinan 250100, China

2.Shandong Univ, Sch Life Sci, 27 Shanda South Rd, Jinan 250100, Shandong, Peoples R China;^Minist Educ, Key Lab Plant Cell Engn & Germplasm Enhancement, Jinan 250100, Shandong, Peoples R China

3.Dongying Economic and Technological Development Zone Dongkai Experimental School, Dongying 257091, China

4.Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China

关键词： Transgenic cotton;Salt tolerance;TsVP;AtNHX1;Seed cotton yield;Ion accumulation

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2018 年 38 卷 2 期

页码：

收录情况： SCI

摘要： Salinity is one of the major abiotic stressors affecting cotton production. The AtNHX1 gene from Arabidopsis thaliana and the TsVP gene from Thellungiella halophila were co-expressed in cotton (cv. GK35) to improve its salt tolerance. Cotton with overexpressed AtNHX1-TsVP genes had higher emergence rates and higher dry matter accumulation under salt stress in the greenhouse and better emergence rates and survival rates in a saline field compared to the WT. More importantly, the cotton with overexpressed AtNHX1-TsVP genes had higher seed cotton yield in the saline field. The growth of transgenic cotton with overexpression of the AtNHX1-TsVP genes may be related to the accumulation of Na+, K+ and Ca2+ in leaves under salt stress. The accumulation of these cations could improve the ability to maintain ion homeostasis and osmotic potential in plant cells under salt stress, thereby conferring cells with higher relative water content and maintaining higher carbon assimilation capacity. These results reveal that overexpression of AtNHX1-TsVP significantly enhances the tolerance of transgenic cotton to high salinity compared to WT. This study aids efforts of breeding salt-tolerant cotton to achieve the strategy of "westward, eastward, northward" in Chinese cotton production.

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