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Self-priming on the plant viral RNAs during reverse transcription-PCR

文献类型： 外文期刊

作者： Zhang, C. 1 ; Wu, H. N. 1 ; Zhang, Y. Q. 1 ; Shen, J. G. 2 ; Li, W. M. 1 ;

作者机构： 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

2.Fujian Entry Exit Inspect & Quarantine Bur, Inspect & Quarantine Technol Ctr, Fuzhou 350001, Peoples R China

关键词： RT-PCR;primer-independent cDNA synthesis;self-priming;plant RNA virus;RT temperature

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The occurrence of the primer-independent cDNA synthesis during RT-PCR analysis of human and animal RNA viruses has been well documented. Conversely, there is scant knowledge about this event in plant RNA viruses. Here we show that the primer-independent cDNA synthesis occurs in all eight different plant RNA viruses tested in this study, suggesting a common phenomenon for RT-PCR analysis of plant RNA viruses. Additional experiments indicate that the event is likely contributed to by RNA self-priming, and can be effectively reduced or eliminated through increasing temperature of the RT reaction.

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