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Isolation and functional analysis of the pathogenicity-related gene VdPR3 from Verticillium dahliae on cotton

文献类型： 外文期刊

作者： Zhang, Ya-Lin 1 ; Li, Zhi-Fang 1 ; Feng, Zi-Li 1 ; Feng, Hong-Jie 1 ; Zhao, Li-Hong 1 ; Shi, Yong-Qiang 1 ; Hu, Xiao-Ping 2 ; Zhu, He-Qin 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China

2.Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China

关键词： Verticillium dahliae;VdPR3;Biological characteristics;Virulence;Extracellular enzyme

期刊名称：CURRENT GENETICS （ 影响因子：3.886； 五年影响因子：3.697 ）

ISSN：

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收录情况： SCI

摘要： The fungal plant pathogen Verticillium dahliae is the causal agent of vascular wilt, a disease that can seriously diminish cotton fiber yield. The pathogenicity mechanism and the identity of the genes that interact with cotton during the infection process still remain unclear. In this study, we investigated the low-pathogenic, non-microsclerotium-producing mutant vdpr3 obtained in a previous study from the screening of a T-DNA insertional library of the highly virulent isolate Vd080; the pathogenicity-related gene (VdPR3) in wild-type strain Vd080 was cloned. Knockout mutants (Delta VdPR3) showed lower mycelium growth and obvious reduction in sporulation ability without microsclerotium formation. An evaluation of carbon utilization in mutants and wild-type isolate Vd080 demonstrated that mutants-lacking VdPR3 exhibited decreased cellulase and amylase activities, which was restored in the complementary mutants (Delta VdPR3-C) to levels similar to those of Vd080. Delta VdPR3 postponed infectious events in cotton and showed a significant reduction in pathogenicity. Reintroduction of a functional VdPR3 copy into Delta VdPR3-C restored the ability to infect cotton plants. These results suggest that VdPR3 is a multifunctional gene involved in growth development, extracellular enzyme activity, and virulence of V. dahliae on cotton.

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