您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses

文献类型： 外文期刊

作者： Cheng, Xiao-Xiao 1 ; Zhao, Li-Hong 1 ; Klosterman, Steven J. 1 ; Feng, Hong-Jie 1 ; Feng, Zi-Li 1 ; Wei, Feng 1 ; Shi, Yong-Qiang 1 ; Li, Zhi-Fang 2 ; Zhu, He-Qin 3 ;

作者机构： 1.State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, 455000, China

2.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China

3.;Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China

4.USDA-ARS, Salinas, California, 93905, United States of America

关键词： Verticillium dahliae;Endochitinase;Prokaryotic expression;Defense response;Conidia germination

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Chitinases function in the digestion of chitin molecules, which are present principally in insects and fungi. In plants, chitinase genes play important roles in defense, and their expression can be triggered in response to both biotic and abiotic stresses. In this study, we cloned and characterized an endochitinase (VDECH) from Verticillium dahliae, strain Vd080. The VDECH coding region consists of 1845 bp with two exons and one 54 by intron, encoding a 615 amino acid protein with the predicted molecular weight (MW) of 63.9 kDa. The VDECH cDNA without signal peptide-encoding region was introduced into pCold-TF vector and the recombinant protein HIS-VDECH with a predicted MW of 114 kDa was expressed. HIS-VDECH showed high tolerance to extreme temperature, exhibiting efficient chitinolytic activity at 50 degrees C. In addition, VDECH triggered typical plant defense responses, including a hypersensitive response, oxidative burst, and elicited increased expression of defense-related genes in both Arabidopsis and cotton. VDECH-treatment of the conidial spores of V. dahliae and Fusarium oxysporum resulted in marked reductions in the germination of these spores in both fungi. After 36 h of incubation with VDECH, the inhibition rate of germination was recorded at 99.57% for V. dahliae, and 96.89% for F. oxysporum. These results provide evidence that VDECH is recognized by the plant to elicit defense responses, and also that VDECH is an effective inhibitor of conidia germination, both of which may be exploited for disease control. (C) 2017 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Transformation of the endochitinase gene Chi67-1 in Clonostachys rosea 67-1 increases its biocontrol activity against Sclerotinia sclerotiorum. Sun, Man-Hong,Zhou, Mo,Li, Shi-Dong. 2017

[2]Cloning, expression and immunocompetence analysis of gene encoding the glutamate dehydrogenase of Streptococcus suis serotype 2. Wei-Jun Yang,Zhi-Biao Yang,Xue-Hui Cai,Yong-Qing Hao,Sheng-Bo Zheng,Dao-Liang Lan,Jiang-Ping Gu,Rui Xing,Cong-Li Yuan,Li Cui,Xiu-Guo Hua. 2010

[3]Comparative Proteomic Analysis of Gossypium thurberi in Response to Verticillium dahliae Inoculation. Weiping Fang,Deyi Xie,Heqin Zhu,Wu Li,Zhenzhen Xu,Lirong Yang,Zhifang Li,Li Sun,Jinxia Wang,Lihong Nie,Zhongjie Tang,Shuping Lv,Fu’an Zhao,Yao Sun,Yuanming Zhao,Jianan Hou,Xiaojie Yang. 2015

[4]OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1. Liu, XQ,Bai, XQ,Qian, Q,Wang, XH,Chen, MS,Chu, CC. 2005

[5]Profiling Gene Expression During Gland Morphogenesis of a Glanded and a Glandless Upland Cotton. Chen, Min,Sun, Quan,Xie, Yong-Fang,Li, Sheng-Wei,Mo, Jian-Chuan,Jiang, Huai-Zhong,Pan, Zheng,Gao, Yun-Ling,Chen, Min,Li, Sheng-Wei,Jiang, Ming-Feng,Yuan, You-Lu,Shi, Yu-Zhen,Ye, Peng-Sheng,Zeng, Hua-Lan.

[6]Wheat resistome in response to barley yellow dwarf virus infection. Chen, Liang,Zhao, Dan,Zhang, Zengyan,Liu, Yan,Wang, Xifeng.

[7]Large-scale identification of wheat genes resistant to cereal cyst nematode Heterodera avenae using comparative transcriptomic analysis. Wu, Du-Qing,Huang, Wen-Kun,Peng, Huan,Wang, Gao-Feng,Cui, Jiang-Kuan,Liu, Shi-Ming,Peng, De-Liang,Li, Zhi-Gang,Yang, Jun,Li, Zhi-Gang,Yang, Jun. 2015

[8]Fungal elicitor protein PebC1 from Botrytis cinerea improves disease resistance in Arabidopsis thaliana. Yang, Xiufen,Zeng, Hongmei,Guo, Lihua,Yuan, Jingjing,Qiu, Dewen. 2014

[9]Isolation and expression analysis of defense-related genes in sorghum-Colletotrichum sublineolum interaction. Li, Lei,Li, Lei,Zhu, Fuyuan,Chu, Apple,Lo, Clive,Liu, Hongjia.

[10]Molecular cloning of a new wheat calreticulin gene TaCRT1 and expression analysis in plant defense responses and abiotic stress resistance. Lin, R. M.,Wang, F. T.,Feng, J.,Xu, S. C.,An, Y. Q.,Xu, Y. F.. 2011

[11]Proteomic Analysis of Differentially Expressed Proteins in Resistant Soybean Leaves after Phakopsora pachyrhizi Infection. Wang, Yun,Yuan, Xiaozhuan,Hu, Hao,Sun, Weihong,Wang, Yun,Liu, Yan,Shan, Zhihui,Zhou, Xinan. 2012

[12]Salicylic acid regulates sugar metabolism that confers tolerance to salinity stress in cucumber seedlings. Dong, Chun-Juan,Wang, Xiao-Li,Shang, Qing-Mao.

[13]Characterization of three pheromone-binding proteins (PBPs) of Helicoverpa armigera (Hubner) and their binding properties. Mei, Xiang-Dong,Zhang, Yong-Jun,Guo, Yu-Yuan,Zhang, Tian-Tao,Feng, Ji-Nian,Guo, Yu-Yuan,Berg, Bente G.. 2012

[14]First Characterization of Sphingomyeline Phosphodiesterase Expression in the Bumblebee, Bombus lantschouensis. Han, L.,Dong, J.,Wang, Y.,Huang, J.,Wu, J.,Han, L.,He, S.. 2017

[15]Molecular genetic characterization of rice seed lipoxygenase 3 and assessment of its effects on seed longevity. Long, Qizhang,Zhang, Wenwei,Wang, Peng,Shen, Wenbiao,Zhou, Tong,Liu, Nannan,Wang, Ren,Jiang, Ling,Huang, Jiexue,Wang, Yihua,Liu, Yuqiang,Wan, Jianmin,Wan, Jianmin.

[16]Cloning, silencing, and prokaryotic expression of strawberry sucrose synthase gene FaSus1. Hua, L. N.,Zang, M.,Wang, S. F.,Shen, Y. Y.,Guo, J. X.,Li, Y. Z..

[17]Detection of the Soybean Allergenic Protein Gly m Bd 28K by an Indirect Enzyme-Linked Immunosorbent Assay. Liu, Bin,Teng, Da,Wang, Xiumin,Wang, Jianhua,Liu, Bin,Teng, Da,Wang, Xiumin,Wang, Jianhua.

[18]Characterization and expression patterns of mannose-binding lectin (MMBL) gene in mulberry (Morus multicaulis) and its prokaryotic expression in E. coli. Li, R.,Chen, D.,Dominic, K.,Li, R.,Liu, L.,Zhao, W.,Li, R.,Wang, T.,Wang, Y.,Li, R.,Chen, D.,Dominic, K.,Li, R.,Liu, L.,Zhao, W.. 2017

[19]Cloning of a caffeoyl-coenzyme A O-methyltransferase from Camellia sinensis and analysis of its catalytic activity. Zhang, Yue,Lv, Hai-peng,Ma, Cheng-ying,Guo, Li,Tan, Jun-feng,Peng, Qun-hua,Lin, Zhi. 2015

[20]Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection. Ge, Junwei,Cui, Xingyang,Shi, Yunjia,Wei, Chengwei,Wen, Shanshan,Xia, Shuang,Zhao, Lili,Chen, Hongyan,Ge, Junwei. 2018

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台