您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A TOPLESS-related gene regulates both male and female sterility in wheat

文献类型： 外文期刊

作者： Wu, Yu 1 ; Guo, Huijun 1 ; Xiong, Hongchun 1 ; Xie, Yongdun 1 ; Zhao, Linshu 1 ; Gu, Jiayu 1 ; Li, Huiyuan 1 ; Ding, Yuping 1 ; Liu, Luxiang 1 ;

作者机构： 1.Chinese Acad Agr Sci, CAEA Res & Dev Ctr Nucl Technol Applicat Irradiat, State Key Lab Crop Gene Resources & Breeding, Natl Engn Lab Crop Mol Breeding,Inst Crop Sci, Beijing, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：4.2； 五年影响因子：4.9 ）

ISSN： 0040-5752

年卷期： 2025 年 138 卷 6 期

页码：

收录情况： SCI

摘要： Wheat yield mainly depends on thousand-grain weight, spike number per unit, and grain number per spike. Since fertility is directly related to seed setting rate and grain number, the discovery of novel genes and alleles affecting male or female sterility is essential for advancing the genetic understanding of wheat fertility. Here we identified a wheat mutant on anther-stigma development (asd), exhibiting both male and female sterility with thin shriveled non-dehiscent anthers that failed to release pollen, as well as visorless stigmas. Cytological examination of asd showed a lack of cutin polymer on anther surfaces and extensive microspore mother cell degeneration leading to pollen deficiency. Using MutMap(+) and exome capture sequencing, we mapped one target gene on chromosome 2D, narrowing it a 1.62-Mb interval, and identifying TraesCS2D03G0987000 (TaTPR1), which encodes a conserved TOPLESS-related protein, as the candidate gene. TaTPR1 harbored a single base mutation (G > A) at the junction of the 6th intron and 7th exon, which disrupted splicing resulting in three transcripts. TaTPR1 expression levels were elevated in mutant anthers and ovaries, with subcellular localization confirming its presence in the nucleus and cytoplasm. Various allelic mutations within TaTPR1 exerted distinct impacts on pollen fertility and stigma development and led to reduced self-setting rates, which verified TaTPR1 to be the target gene. This study identified a crucial novel gene affecting both male and female sterility in wheat, which offers new insights into fertility mechanisms.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台