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Development of an Agrobacterium-mediated CRISPR/Cas9 system in pea (Pisum sativum L.)

文献类型： 外文期刊

作者： Li, Guan 1 ; Liu, Rong 1 ; Xu, Rongfang 2 ; Varshney, Rajeev K. 3 ; Ding, Hanfeng 4 ; Li, Mengwei 1 ; Yan, Xin 1 ; Huang, Shuxian 1 ; Li, Juan 2 ; Wang, Dong 1 ; Ji, Yishan 1 ; Wang, Chenyu 1 ; He, Junguang 5 ; Luo, Yingfeng 6 ; Gao, Shenghan 6 ; Wei, Pengcheng 2 ; Zong, Xuxiao 1 ; Yang, Tao 1 ;

作者机构： 1.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Inst Crop Sci, Beijing 100081, Peoples R China

2.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui Prov, Hefei 230031, Anhui, Peoples R China

3.Murdoch Univ, Food Futures Inst, State Agr Biotechnol Ctr, Ctr Crop & Food Innovat, Murdoch, WA 6150, Australia

4.Shandong Acad Agr Sci, Inst Crop Germplasm Resources, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Shandong, Peoples R China

5.Zhejiang Xinnan Chem Ind Grp Co Ltd, Hangzhou 311300, Zhejiang, Peoples R China

6.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China

关键词： Agrobacterium-mediated transformation; CRISPR/Cas9; Pea; Genome editing

期刊名称：CROP JOURNAL （ 影响因子：6.6； 五年影响因子：6.5 ）

ISSN： 2095-5421

年卷期： 2023 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Pea (Pisum sativum L.) is an annual cool-season legume crop. Owing to its role in sustainable agriculture as both a rotation and a cash crop, its global market is expanding and increased production is urgently needed. For both technical and regulatory reasons, neither conventional nor transgenic breeding tech-niques can keep pace with the demand for increased production. In answer to this challenge, CRISPR/ Cas9 genome editing technology has been gaining traction in plant biology and crop breeding in recent years. However, there are currently no reports of the successful application of the CRISPR/Cas9 genome editing technology in pea. We developed a transient transformation system of hairy roots, mediated by Agrobacterium rhizogenes strain K599, to validate the efficiency of a CRISPR/Cas9 system. Further opti-mization resulted in an efficient vector, PsU6.3-tRNA-PsPDS3-en35S-PsCas9. We used this optimized CRISPR/Cas9 system to edit the pea phytoene desaturase (PsPDS) gene, causing albinism, by Agrobacterium-mediated genetic transformation. This is the first report of successful generation of gene-edited pea plants by this route. (c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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