您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Inhibitory effects of Lactobacillus brevis on Aspergillus westerdijkiae and antifungal compounds identification

文献类型： 外文期刊

作者： Li, Li 1 ; Yang, Bolei 1 ; Yang, Shuo 2 ; Tian, Xiaoyu 1 ; Gao, Yuan 1 ; Song, Jun 3 ; Wang, Haifeng 3 ; Li, Tiejun 3 ; Xing, Fuguo 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Food Sci & Technol, Minist Agr & Rural Affairs, Key Lab Agroprod Qual & Safety Control Storage & T, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Feed Res Inst, Beijing 100081, Peoples R China

3.Dongfeng Country Sika Deer Ind Dev Serv Ctr, Jilin 136300, Peoples R China

关键词： Ochratoxin A; Aspergillus westerdijkiae; Lactobacillus brevis 8-2B; Antifungal compounds identification; Transcriptome; Inhibition mechanism

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：7.0； 五年影响因子：6.9 ）

ISSN： 0925-5214

年卷期： 2024 年 214 卷

页码：

收录情况： SCI

摘要： Ochratoxin A (OTA), a secondary metabolite produced by Aspergillus and Penicillium , seriously contaminates grapes and its products. Inhibiting the growth of OTA-producing fungi is the best approach to reduce OTA contamination in grapes. This study was to reveal the inhibition mechanism of Lactobacillus brevis 8-2B on Aspergillus westerdijkiae. Research has found that L. brevis 8-2B could inhibit the germination, growth, and OTA synthesis of A. westerdijkiae . The antifungal activity of L. brevis 8-2B is attributed to the synergistic effect of acetic acid, lactic acid, citric acid, longifolene, methyl salicylate, 3-phenyl-2-propenal, nystatin, and itraconazole. The inhibitory effect of L. brevis 8-2B on A. westerdijkiae was the synergistic effect of these active substances. Ultrastructure observation and flow cytometry results showed that the mycelia structure, cell membrane and cell wall of A. westerdijkiae were disrupted, the cells showed apoptosis, and the ergosterol content in the cells was reduced. Transcriptome analysis revealed that the expression levels of ribosomal biosynthesis genes and cell wall chitin synthesis genes in inhibited A. westerdijkiae were up -regulated, while the expression of chitin degrading enzyme genes was down -regulated to enhance its primary growth and development. In addition, L. brevis 8-2B also inhibits the expression of amino acid synthesis and glycolysis genes in A. westerdijkiae , reducing the generation of secondary metabolite precursors such as NRPS and PKS, thereby inhibiting OTA biosynthesis. On grapes, L. brevis 8-2B could inhibit the infection of A. westerdijkiae, and prolong its shelf life. This study provides new insights for the research and development of novel agent to control OTA contamination.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台