您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Nuclear shuttling of CDC4 mediated broad-spectrum antiviral activity against diverse coronaviruses

文献类型： 外文期刊

作者： Li, Mingwei 1 ; Wu, Yang 1 ; Tian, Jin 1 ; Yang, Qian 2 ; Gao, Mingze 1 ; Wang, Yongrui 1 ; Wang, Xuepeng 1 ; Zhang, Ju 1 ; Pan, Yudi 1 ; Shi, Hongyan 1 ; Shi, Da 1 ; Zhang, Xin 1 ; Chen, Jianfei 1 ; Guo, Longjun 1 ; Feng, Li 1 ;

作者机构： 1.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, 678 Haping Rd, Harbin 150069, Peoples R China

2.Harbin Med Univ, Sch Basic Med Sci, Lab Med Genet, Harbin, Peoples R China

关键词： Coronavirus; CDC4; nuclear shuttling; broad-spectrum; antiviral activity

期刊名称：EMERGING MICROBES & INFECTIONS （ 影响因子：7.5； 五年影响因子：7.2 ）

ISSN：

年卷期： 2025 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Pandemics of coronavirus (CoV)-related infection have been a major issue since the outbreaks of SARS, MERS and COVID-2019 in the past decades, leading a substantial threat to public health. Porcine deltacoronavirus (PDCoV), a new swine coronavirus, causes enteropathogenic disease characterized by acute diarrhoea, vomiting and dehydration in suckling piglets and poses potential risks of cross-species transmission. Here we reveal a novel function of CDC4 protein in restricting PDCoV infection. Ectopic expression of CDC4 suppresses PDCoV replication, whereas knockdown of CDC4 expression enhances PDCoV infection. Importantly, it was revealed that PDCoV encoded nucleocapsid (N) was involved in CDC4 nuclear-cytoplasmic shuttling, which was critical for CDC4 to exert the antiviral activity against PDCoV replication. Mechanistically, PDCoV N protein was detected to specifically interact with RIG-I to antagonize RIG-I-like receptor (RLR)-mediated IFN-beta production, leading to disruptions of host innate immune defense. Meanwhile, CDC4 was proved to interact with PDCoV N protein and disrupted the interaction between PDCoV N and RIG-I, resulting in alleviated antagonism of IFN-beta production mediated by PDCoV N. Similarly, a broad-spectrum inhibitory effects of CDC4 on N mediated antagonism were confirmed by the shared mechanisms among the different coronaviruses from Coronaviridae family, such as transmissible gastroenteritis virus (TGEV) from Alphacoronavirus (alpha-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from Betacoronavirus (beta-CoV). Therefore, a novel antiviral role of CDC4 was elucidated that CDC4 competes binding with CoVs N proteins to suppress CoVs N mediated antagonism of RLR associated signalling pathway in the context of diverse coronavirus infections.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台