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Selenium Treatment Alleviates the Inhibition Caused by Nep-L Gene Knockdown in Silkworm (Bombyx mori)

文献类型： 外文期刊

作者： Hong, Ting-Ting 1 ; Hu, Fei 1 ; Ge, Wen-Jie 1 ; Zhang, Rui 1 ; Du, Juan 2 ; Thakur, Kiran 1 ; Tang, Shun-Ming 3 ; Wei, Zhao-Jun 1 ;

作者机构： 1.Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230009, Peoples R China

2.North Minzu Univ, Sch Biol Sci & Engn, Yinchuan 750021, Peoples R China

3.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural Biol & Biotechnol, Zhenjiang 212003, Peoples R China

4.Chinese Acad Agr Sci, Sericultural Res Inst, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr, Zhenjiang 212018, Peoples R China

关键词： Bombyx mori; RNAi; Neutral endopeptidase (Nep); Selenium, Expression

期刊名称：BIOLOGICAL TRACE ELEMENT RESEARCH （ 影响因子：3.4； 五年影响因子：3.7 ）

ISSN： 0163-4984

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Recent studies have emphasized the beneficial effects of 50 mu M selenium (Se) on the growth and development of the silkworm, Bombyx mori; however, less is known about its underlying mechanism. To unravel the effect of 50 mu M Se on the silkworms with neutral endopeptidase 24.11-like gene (NEP-L) knockdown, we injected small interfering RNA (siRNA) into the body cavity of silkworms. Phenotypic characteristics, mRNA expression of the Nep-L gene, and enriched Se content were evaluated in silkworms from each treatment group. After injecting Nep-L siRNA, the body weight, cocoon quality (cocoon weight, cocoon shell weight, and cocoon shell ratio), and egg production of silkworms were significantly reduced, without any significant effect on egg laying number. However, Se treatment could significantly alleviate the inhibition of body weight, and cocoon quality, without significant effects on egg laying number and production. In addition, the gene knockdown increased Se content in the B. mori. On the molecular level, the targeted Nep-L gene was inhibited significantly by siRNA interference, essentially with the strongest effect at 24 h after RNAi, followed by steady recovery. Among the three fragments, the siRNA of Nep-L-3 was the most effective in interfering with target gene expression. Nep-L gene showed the highest expression in Malpighian tubules (MTs). Both at the phenotypic and genotypic levels, our results show that Nep-L knockdown can exert a significant inhibitory effect on silkworms, and 50 mu M Se can reverse the negative effect, which provides a practical prospect for strengthening the silkworm food industry.

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