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Development of an on-site diagnostic LAMP assay for rapid differentiation of the invasive pest Phthorimaea absoluta (Meyrick) using insect tissues

文献类型： 外文期刊

作者： Yang, Li-Feng 2 ; Liu, Ya-Ge 2 ; Tao, Yun-Li 2 ; Zhang, Wan-Min 4 ; Li, Jian-Yong 5 ; Chi, Sheng-Qi 2 ; Zhang, Gui-Fen 6 ; Chu, Dong 1 ;

作者机构： 1.Qingdao Agr Univ, Coll Plant Hlth & Med, Shandong Engn Res Ctr Environm friendly Agr Pest M, Qingdao 266109, Peoples R China

2.Qingdao Agr Univ, Coll Plant Hlth & Med, Shandong Engn Res Ctr Environm friendly Agr Pest M, Qingdao, Peoples R China

3.Qingdao Agr Univ, Shandong Prov Ctr Bioinvas & Ecosecur, Qingdao, Peoples R China

4.Liaoning Technol Ctr Green Agr, Dept Pest Predict & Management, Shenyang, Peoples R China

5.Tech Ctr Jinan Customs Dist, Jinan, Peoples R China

6.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Key Lab Integrated Pest Management Crop, Minist Agr & Rural Affairs, Beijing 100193, Peoples R China

关键词： loop mediated isothermal amplification (LAMP); Phthorimaea absoluta; invasive species; on-site diagnostics

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）

ISSN： 1526-498X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： BACKGROUND The tomato leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a destructive invasive pest that originated in South America and has spread within China since 2017. A rapid method for on-site identification of P. absoluta is urgently needed for interception of this pest across China. RESULTS We developed a loop-mediated isothermal amplification (LAMP) technique to differentiate P. absoluta from Liriomyza sativae, Chromatomyia horticola, and Phthorimaea operculella using extracted genomic DNA, which was then refined to create an on-site LAMP diagnostic method that can be performed under field conditions without the need for laboratory equipment. CONCLUSION In the present research, we developed an on-site diagnostic method for rapid differentiation of P. absoluta from other insects with similar morphology or damage characteristics in China.

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