您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Dual function of the Tuta absoluta 3-phosphoinositide-dependent protein kinase-1 in pupa ecdysis and adult reproduction

文献类型： 外文期刊

作者： Yan, Shu-Yan 1 ; Song, Jia-Hui 2 ; Yang, Wen-Jia 2 ; Liu, Wan-xue 1 ; Zhang, Gui-Fen 1 ; Wan, Fang-Hao 1 ; Desneux, Nicolas 3 ; Zhang, Yi-Bo 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Key Lab Invas Alien Species Control,Minist Agr & R, Beijing 100193, Peoples R China

2.Guiyang Univ, Coll Biol & Environm Engn, Key Lab Surveillance & Management Invas Alien Spec, Guizhou Educ Dept, Guiyang 550005, Peoples R China

3.Univ Cote Azur, INRAE, CNRS, UMR ISA, F-06000 Nice, France

关键词： Tuta absoluta; PDK; RNA interference; Ecdysis; Reproduction; Gene regulation

期刊名称：JOURNAL OF PEST SCIENCE （ 影响因子：4.1； 五年影响因子：5.0 ）

ISSN： 1612-4758

年卷期： 2025 年 98 卷 2 期

页码：

收录情况： SCI

摘要： Phosphoinositide-dependent kinase PDK, a key component of the insulin signaling pathway, plays an important role in the regulation of insect growth and development. However, the functional significance of the PDK gene in Tuta absoluta, a destructive invasive tomato leaf miner, remains unknown. In this study, we identified and characterized a PDK gene (designated TaPDK) and analyzed its physiological roles in molting and reproduction of T. absoluta. Our results showed that the TaPDK expression levels were elevated in the 5- and 6-day-old pupae before ecdysis and declined immediately after eclosion. TaPDK was predominantly expressed in the head, midgut and abdomen. When the TaPDK was knockdown, the larval-pupal molting and female reproduction were severely affected. Injecting dsRNA of TaPDK into 2-day-old female pupae resulted in severe phenotypic changes (cuticular shrinkage) in 31.29% of pupae, ultimately leading to death. Knockdown of TaPDK significantly reduced the 20E titer and chitin content and downregulated the expression levels of genes involved in the 20E pathway and chitin metabolism. Further investigation revealed that the suppression of TaPDK impaired ovarian development, resulting in significantly reduced fecundity and hatchability. In addition, the JH titer and vitellogenin content of TaPDK-deficient females were reduced, and the expression of Vg, VgR, and JH signaling pathway genes were significantly decreased. Collectively, our study provides a deep insight into the PDK-mediated regulation of pupal ecdysis and female reproduction in T. absoluta.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台