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Uridine diphosphate glucosyltransferase is vital for fenpropathrin resistance in Bombyx mori (Lepidoptera)

文献类型： 外文期刊

作者： Zheng, Kai-yi 1 ; Zhang, Xiao-ying 1 ; Lisan, Fasihul 1 ; Lai, Wen-Qin 1 ; Zhang, Qiang 1 ; Lv, Jun-li 1 ; Lu, Zhan-peng 1 ; Qin, Sheng 1 ; Sun, Xia 1 ; Zhang, Shang-zhi 3 ; Wang, Xue-yang 1 ; Dai, Li-shang 4 ; Li, Mu-wang 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural & Anim Biotechnol, Zhenjiang, Peoples R China

2.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Sericultural Sci Res Ctr, Key Lab Silkworm & Mulberry Genet Improvement, Zhenjiang, Peoples R China

3.Hunan Univ Humanities Sci & Technol, Coll Agr & Biotechnol, Loudi, Peoples R China

4.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou, Peoples R China

5.Jiangsu Univ Sci & Technol, Sch Biotechnol, Zhenjiang 212100, Jiangsu, Peoples R China

6.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Peoples R China

关键词： Bombyx mori; fenpropathrin; pest management; resistance mechanism; UGT

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：2.6； 五年影响因子：2.9 ）

ISSN： 0962-1075

年卷期： 2024 年

页码：

收录情况： SCI

摘要： The silkworm (Bombyx mori) is an important model lepidopteran insect and can be used to identify pesticide resistance-related genes of great significance for biological control of pests. Uridine diphosphate glucosyltransferases (UGTs), found in all organisms, are the main secondary enzymes involved in the metabolism of heterologous substances. However, it remains uncertain if silkworm resistance to fenpropathrin involves UGT. This study observes significant variations in BmUGT expression among B. mori strains with variable fenpropathrin resistance post-feeding, indicating BmUGT's role in fenpropathrin detoxification. Knockdown of BmUGT with RNA interference and overexpression of BmUGT significantly decreased and increased BmN cell activity, respectively, indicating that BmUGT plays an important role in the resistance of silkworms to fenpropathrin. In addition, fenpropathrin residues were significantly reduced after incubation for 12 h with different concentrations of a recombinant BmUGT fusion protein. Finally, we verified the conservation of UGT to detoxify fenpropathrin in Spodoptera exigua: Its resistance to fenpropathrin decreased significantly after knocking down SeUGT. In a word, UGT plays an important role in silkworm resistance to fenpropathrin by directly degrading the compound, a function seen across other insects. The results of this study are of great significance for breeding silkworm varieties with high resistance and for biological control of pests.

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