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Induction of Apoptosis in HeLa Cells by a Novel Peptide from Fruiting Bodies of Morchella importuna via the Mitochondrial Apoptotic Pathway

文献类型： 外文期刊

作者： Xiong, Chuan 1 ; Li, Ping 1 ; Luo, Qiang 2 ; Phan, Chia Wei 3 ; Li, Qiang 5 ; Jin, Xin 2 ; Huang, Wenli 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Biotechnol & Nucl Technol Res Inst, Chengdu 610061, Peoples R China

2.Chongqing Med Univ, Affiliated Hosp 2, Chongqing 400010, Peoples R China

3.Univ Malaya, Fac Pharm, Dept Pharmaceut Life Sci, Kuala Lumpur 50603, Malaysia

4.Univ Malaya, Mushroom Res Ctr, Kuala Lumpur 50603, Malaysia

5.Chengdu Univ, Coll Food & Bioengn, Minist Agr & Rural Affairs, Key Lab Coarse Cereal Proc, Chengdu 610106, Peoples R China

期刊名称：EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE （ 影响因子：2.63； 五年影响因子：2.846 ）

ISSN： 1741-427X

年卷期： 2021 年 2021 卷

页码：

收录情况： SCI

摘要： Morels (Morchella spp.) are a genus of edible fungi with important economic and medicinal value. In this study, a novel peptide (MIPP) was extracted from the fruiting bodies of Morchella importuna using gel filtration chromatography. Structural analysis showed that the molecular mass of MIPP is 831 Da, and it has a simple amino acid sequence: Ser-Leu-Ser-Leu-Ser-Val-Ala-Arg. To explore the antitumor activity of MIPP, the effect of MIPP on HeLa cell apoptosis and the underlying preventative mechanisms were investigated. Results showed that MIPP reduced the viability of HeLa cells in a concentration-dependent manner. TUNEL analysis and flow cytometric examination showed that MIPP decreased cell proliferation via a mitochondrial-dependent pathway, as manifested by downregulation of Bcl-2/Bax, promotion of the movement of cytochrome C from the mitochondria to the cytoplasm, and triggering of caspase-9 and caspase-3. Therefore, MIPP may be a promising tumor-preventive agent, especially in human cervical cancer.

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