文献类型： 外文期刊

作者： Chen, Wei-Gang ¹ ; Li, Li ¹ ; Dai, Guo-Dong ¹ ; Wu, Yao-Dong ¹ ; Shumuye, Nigus Abebe ⁸ ; Amuda, Tharheer Oluwashola ¹ ; Tian, Xue-Qi ¹ ; Gao, Sheng-Zhi ⁹ ; Hou, Xue-Yi ² ; Zhang, Nian-Zhang ¹ ; Tian, Wen-Jun ¹ ; Zhang, Lin-Sheng ¹ ; Li, Le ¹ ; Zhang, Fu-Heng ¹¹ ; Guo, Min-Kuan ⁵ ; Li, Wen-Dong ⁵ ; Zhonggecao, Tao ¹⁰ ; Zhaxilamao, Bao-Quan ¹⁰ ; Gazangzhuoma, Hong ¹⁰ ; Doukao, Hong-Bin ¹⁰ ; Yang, Tao ¹² ; Fu, Bao-Quan ¹ ; Yin, Hong ¹ ; Yan, Hong-Bin ¹ ; Jia, Wan-Zhong ¹ ;

作者机构： 1.Lanzhou Univ, Chinese Acad Agr Sci, Coll Vet Med, State Key Lab Anim Dis Control & Prevent, Lanzhou 730046, Gansu, Peoples R China

2.Chinese Acad Agr Sci, Natl Para Reference Lab Anim Echinococcosis, Lanzhou 730046, Gansu, Peoples R China

3.Chinese Acad Agr Sci, Gansu Prov Res Ctr Basic Disciplines Pathogen Biol, Lanzhou 730046, Gansu, Peoples R China

4.Chinese Acad Agr Sci, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China

5.Chinese Acad Agr Sci, Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China

6.Chinese Acad Agr Sci, Key Lab Ruminant Dis Prevent & Control West, Minist Agr & Rural Affairs, Lanzhou 730046, Gansu, Peoples R China

7.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Lanzhou 730046, Gansu, Peoples R China

8.Mekelle Univ, Coll Vet Sci, Dept Vet Clin Med & Epidemiol, POB 2084,Kalamino Campus, Mekelle, Ethiopia

9.Gansu Prov Ctr Anim Dis Prevent & Control, Lanzhou 730046, Gansu, Peoples R China

10.Luqu Ctr Anim Dis Prevent & Control, Luqu 747200, Gansu, Peoples R China

11.Maqu Ctr Anim Dis Prevent & Control, Maqu 747300, Gansu, Peoples R China

12.Xizang Ctr Anim Dis Prevent & Control, Lhasa 850000, Tibet Autonomou, Peoples R China

13.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

关键词： Echinococcus shiquicus; Plateau zokor; Intermediate host; Qinghai-Tibet Plateau; Genetic diversity

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.0； 五年影响因子：2.1 ）

ISSN： 0932-0113

年卷期： 2025 年 124 卷 6 期

页码：

收录情况： SCI

摘要： Echinococcosis is a severe parasitic zoonosis worldwide distribution, caused by the genus Echinococcus. One of its recently identified species, Echinococcus shiquicus, is believed to be endemic to the Qinghai-Tibet plateau in China. Research has confirmed that Tibetan foxes are the definitive hosts for E. shiquicus, while plateau pikas (Ochotona curzoniae) serve as intermediate hosts. In this study, cyst containing protoscolices was first observed in the lung of plateau zokor (Eospalax baileyi) in the Tibetan Autonomous Prefecture of Gannan, Gansu Province, China. This observation was confirmed using complete sequences of the cox1 and nad1 genes. We also characterized the cysts and examined the genetic diversity of Echinococcus shiquicus collected from the Qinghai-Tibet Plateau region, which includes Gansu Province, Qinghai Province, and the Tibet Autonomous Region. Phylogenetic trees and haplotype analyses for the isolates were constructed using the maximum likelihood method in MEGA 7.0.26 and DnaSP v6 software, based on the complete cox1 (1608 bp) and nad1 (897 bp) gene sequences, as well as a concatenated dataset of cox1-nad1 (2505 bp). The cyst containing protoscolices in the lung of plateau zokor was identified as Echinococcus shiquicus. The prevalence of E. shiquicus infection in plateau pikas in Luqu and Maqu counties in Gansu province was found to be 2.38% (3/126) and 3.36% (10/298), respectively, while in plateau zokors, it was 1.61% (1/62). Haplotype analysis, incorporating sequences from previous studies, revealed the presence of 17 haplotypes, indicating significant haplotype diversity in the study areas. The neutrality indices were Tajima's D = - 1.438 (P > 0.05) and Fu's Fs = - 3.169 (P > 0.05). This study provides important insights into Echinococcus shiquicus and its associations with plateau zokors and plateau pikas in the Qinghai-Tibet Plateau. We identified plateau zokors as a new natural intermediate host for E. shiquicus, emphasizing the complexity of its transmission dynamics and host interactions within this unique ecosystem. The observed morphological and developmental state differences in cysts between the liver and lung suggest that presence of tissue specificity in different organ. Additionally, the analysis of genetic diversity revealed high intraspecific variability, indicating a complex population structure influenced by various ecological factors.