Multi-Time Point Transcriptome Analysis and Functional Validation Revealed Bol4CL41 Negatively Regulates Black Rot Resistance in Cabbage
文献类型： 外文期刊
作者： Ma, Hongxue 1 ; Deng, Siping 2 ; Kong, Congcong 2 ; Zhang, Yulun 2 ; Zhao, Tong 2 ; Ji, Jialei 2 ; Wang, Yong 2 ; Zhang, Yangyong 2 ; Zhuang, Mu 2 ; Yang, Limei 2 ; Lebedeva, Marina 4 ; Taranov, Vasiliy 4 ; Artemyeva, Anna M. 5 ; Fang, Zhiyuan 1 ; Yu, Jingquan 1 ; Hu, Zhangjian 1 ; Lv, Honghao 2 ;
作者机构： 1.Zhejiang Univ, Coll Agr & Biotechnol, Dept Hort, Hangzhou 310058, Peoples R China
2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China
3.China Agr Univ, Dept Plant Pathol, Beijing 100083, Peoples R China
4.Russian Acad Sci, All Russia Res Inst Agr Biotechnol, Moscow 119991, Russia
5.NI Vavilov All Russian Inst Plant Genet Resources, Fed Res Ctr, St Petersburg 190000, Russia
关键词：
multi-time point transcriptome; cabbage; black rot;
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 13 期
页码：
收录情况： SCI
摘要： 4-coumarate-CoA ligase (4CL) plays a crucial role in the phenylpropanoid metabolic pathway and is a key enzyme involved in plant growth and stress responses. Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is a major bacterial disease affecting the production of global cruciferous crop-like cabbage (Brassica oleracea var. capitata). However, the role of 4CL genes in cabbage resistance to black rot remains unclear. In this study, transcriptome sequencing was conducted using resistant cabbage MY and susceptible cabbage LY at 0, 6, 24, and 48 h post-inoculation. KEGG analysis identified the enrichment of the phenylpropanoid biosynthesis pathway, and significant expression changes of 4CL genes were determined through the expression heat map. Further genome-wide analysis revealed 43 Bol4CL gene family members on the cabbage genome distributed across nine chromosomes. Gene structure and protein motif analysis revealed similarities in motifs within the same evolutionary branch, but variations in gene structure. A combination of Bol4CL gene expression profiles and differentially expressed genes (DEGs) from the transcriptome identified Bol4CL41 as a key gene for further study. Inoculation of overexpressed Bol4CL41 T2 generation stably expressed cabbage seedlings demonstrated significantly larger lesion areas compared to wild type cabbage, indicating that Bol4CL41 negatively regulates resistance to black rot in cabbage. The analysis of multi-time point transcriptomes in cabbage and the functional study of the Bol4CL gene family enhance our understanding of the mechanisms underlying plant disease resistance. This provides compelling evidence and experimental support for elucidating the mechanisms of black rot resistance in cabbage.
- 相关文献
作者其他论文 更多>>
-
Structural insights into the mechanism of phosphate recognition and transport by XPR1
作者：Zhang, Wenhui;Chen, Yanke;Guan, Zeyuan;Tang, Meng;Du, Zhangmeng;Zhang, Jie;Cheng, Meng;Zuo, Jiaqi;Liu, Yan;Wang, Qiang;Liu, Yanjun;Zhang, Delin;Yin, Ping;Ma, Ling;Liu, Zhu;Wang, Yong;Liu, Zhu
关键词：
-
Putative Transformation Mechanism of γ-
l -Glutamyl-S-Allyl-l -Cysteine during the Processing of Black Garlic作者：Liu, Pingxiang;Wu, Peng;Bi, Jingxiu;Jiang, Yuying;Gao, Rui;Zhao, Tong;Yuan, Xuexia;Hao, Haining;Wang, Yutao;Weng, Rui;Chen, Jing
关键词：gamma-l-glutamyl-S-allyl-l-cysteine; thermal processing; Maillard reaction; enzymatic hydrolysis; transformationmechanism
-
Identification of Novel 58-5p and SREBF1 Interaction and Effects on Apoptosis of Ovine Ovarian Granulosa Cell
作者：Yang, Ruochen;Wang, Yong;Yue, Sicong;Liu, Yueqin;Zhang, Yingjie;Duan, Chunhui;Yang, Ruochen;Wang, Yong
关键词：ovine ovarian GCs; PRL; apoptosis; novel 58-5p; SREBF1
-
Genome-Wide Scans for Selection Signatures in Ningxia Angus Cattle Reveal Genetic Variants Associated with Economic and Adaptive Traits
作者：Yin, Haiqi;Wang, Yaxuan;Peng, Ruiqi;Wang, Yahui;Zhao, Tong;Zheng, Caihong;Xu, Lingyang;Gao, Xue;Gao, Huijiang;Li, Junya;Wang, Zezhao;Zhang, Lupei;Feng, Yuan;Wang, Yu;Jiang, Qiufei;Zhao, Jie;Zhang, Juan;Chen, Yafei
关键词：Angus cattle; whole-genome resequencing; selection signatures; iHS; immune-related gene; economic trait
-
Rapid introgression of the clubroot resistance gene CRa into cabbage skeleton inbred lines through marker assisted selection
作者：Zhang, Na;Zhu, Mingzhao;Qiu, Yuting;Fang, Zhiyuan;Zhuang, Mu;Zhang, Yangyong;Lv, Honghao;Ji, Jialei;Yang, Limei;Wang, Yong;Zhu, Mingzhao;Hou, Xilin
关键词：Clubroot; Breeding; Backcross; Marker assisted selection
-
Enhancing drying efficiency and physicochemical & antioxidant properties of germinated brown rice through microwave incorporation
作者：Tie, Xiaoyu;Huo, Da;Wan, Lina;Zhao, Dan;An, Nannan;Li, Dong;Wang, Lijun;Wang, Yong
关键词：Drying methods; Drying characteristics; Starch gelatinization; Antioxidant activity
-
Variation in a single allele drives divergent yield responses to elevated CO2 between rice subspecies
作者：Liu, Yunlong;Zhang, Siyu;Shen, Chengbo;Ding, Yanfeng;Li, Shan;Jiang, Yu;Liu, Yunlong;Zhang, Siyu;Qian, Haoyu;Shen, Chengbo;Wang, Songhan;Liu, Zhenghui;Li, Ganghua;Ding, Yanfeng;Li, Shan;Jiang, Yu;Liu, Yunlong;Qian, Haoyu;Wang, Songhan;Liu, Zhenghui;Li, Ganghua;Ding, Yanfeng;Jiang, Yu;Hu, Shuijin;Zhang, Weijian;Wang, Yong;Huang, Shan;Wang, Yong;Huang, Shan;Fu, Xiangdong;Fu, Xiangdong;van Groenigen, Kees Jan
关键词：
