文献类型： 外文期刊

作者： Yuan, Xiangxiang ¹ ; Han, Xiaoge ¹ ; Kong, Xinping ¹ ; Hou, Linjing ¹ ; Wei, Kelong ⁵ ; Chen, Mingtang ⁵ ; Zhang, Weiyu ¹ ; Di, Wenda ¹ ;

作者机构： 1.Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Peoples R China

2.Guangxi Coll & Univ Key Lab Prevent & Control Anim, Nanning 530004, Peoples R China

3.Guangxi Zhuang Autonomous Reg Engn Res Ctr Vet Bio, Nanning 530004, Peoples R China

4.Guangxi Key Lab Anim Reprod Breeding & Dis Control, Nanning 530004, Peoples R China

5.Chinese Acad Agr Sci, Guangxi Buffalo Res Inst, Nanning 530001, Peoples R China

关键词： Fasciola gigantica; Buffalo; cell proliferation; immunomodulatory; PBMCs

期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2023 年 13 卷 4 期

页码：

收录情况： SCI

摘要： Simple Summary The 22nd chromatography component (F22) of the Fasciola gigantica excretory-secretory products (FgESP) shows better diagnostic value than the FgESP, but its immunomodulatory function and potential as a molecular vaccine candidate are unknown. Thus, the effect of F22 on the mitogen-induced proliferation of buffalo peripheral blood mononuclear cells (PBMCs) in the innate immune response was preliminarily studied, and its components were also explored. The results indicated that F22 significantly decreased the proliferation of PBMCs stimulated with mitogen. Two hundred and sixteen were identified as components of the F22, and these included eighty-six proteins present in more than one pathway and some with robust immunomodulatory ability. Further studies should be performed to investigate the immunomodulatory function of F22 in the adaptive immune response, and the components of F22 can be further studied as potential vaccine candidate molecules. The 22nd chromatography component (F22) of the Fasciola gigantica excretory-secretory products (FgESP) shows better diagnostic value than the FgESP, and diagnostic methods based on F22 have also been established. Thus, exploring its immunomodulatory function and potential as a molecular vaccine candidate is attractive. In the present study, the effect of F22 on the mitogen-induced proliferation of buffalo peripheral blood mononuclear cells (PBMCs) in the innate immune response was preliminarily studied using the FgESP as a control. PBMCs were incubated with concanavalin A (ConA) and phytohemagglutinin (PHA) at optimal (1 mu g/well) or suboptimal (0.25 mu g/well) doses coupled with FgESP and F22 at different doses (1-16 mu g/well). Cell proliferation was then assessed by microenzyme reaction colorimetry (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay). In addition, the components of F22 were also explored by mass spectrometry and then subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis to infer their functions. The results indicated that FgESP decreased the proliferation of PBMCs stimulated with ConA and PHA at specific doses, whereas F22 significantly decreased the proliferation of PBMCs stimulated with ConA and PHA at both optimal and suboptimal doses (p < 0.05). Two hundred and sixteen proteins were identified in F22, and these included 86 proteins that could be assigned to more than one pathway and some with robust immunomodulatory ability. Further studies should be performed to investigate the immunomodulatory function of F22 in the adaptive immune response, and the components of F22 can be further studied as potential vaccine candidate molecules.