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Culture filtrates derived from endophytes Epicoccum spp. confer resistance against tobacco mosaic virus in Nicotiana benthamiana

文献类型： 外文期刊

作者： An, Qi 1 ; Nzabanita, Clement 2 ; Zhang, Lihang 2 ; Wang, Yanfei 2 ; Wang, Shuangchao 2 ; Guo, Lihua 2 ; Zhao, Yan 1 ;

作者机构： 1.Inner Mongolia Agr Univ, Coll Grassland Sci, Hohhot 010018, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

关键词： Biocontrol; Epicoccum spp.; Nicotiana benthamiana; Tobacco mosaic virus; LC-MS

期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：3.3； 五年影响因子：3.2 ）

ISSN： 0885-5765

年卷期： 2025 年 138 卷

页码：

收录情况： SCI

摘要： Tobacco mosaic virus (TMV) is a plant viral disease significantly affecting the losses of many economically important crops, and no active viricide is currently available for agricultural use. Herein, the efficacy of culture filtrates derived from fungal endophytes Epicoccum layuense strain J4-3 and E. nigrum strain 38L1, respectively isolated from symptomless stem of wheat and leaf of maize, was evaluated in reducing TMV infection in Nicotiana benthamiana. In doing so, dual foliar treatment was carried out 24 h before TMV inoculation under greenhouse conditions. Tracking the spread of green fluorescence protein-tagged TMV (TMV-GFP) in N. benthamiana plants revealed that the culture filtrates restricted the initial establishment of virus infection. Notably, quantitative reverse transcription polymerase chain reaction (qRT-PCR) results indicated a notable decrease in TMV accumulation in plants treated with endophyte's filtrates compared to the untreated controls. Additionally, using liquid chromatography-mass spectrometry (LC-MS), we identified a total of 8366 metabolites, including 569 differential metabolites based on variable importance in projection (VIP) > 1 and P < 0.05. Interestingly, previously reported metabolites with anti-TMV activity namely 13-Docosenamide, Chondroitin sulfate, and Melatonin were identified in culture filtrates of the strains J4-3 and 38L1. This is the first study depicting culture filtrates derived from fungal endophytes Epicoccum spp. to extenuate TMV virulence in N. benthamiana.

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