The P-type ATPase gene AHA5 is involved in proanthocyanidins accumulation in Medicago truncatula
文献类型： 外文期刊
作者： Jiang, Wenbo 1 ; Yan, Yinuo 1 ; Yue, Shiyao 1 ; Wei, Jiebing 1 ; Li, Wenxiang 1 ; Liang, Yanxia 1 ; Xu, Mengrong 1 ; Xia, Yaying 1 ; Yi, Dengxia 1 ; Wang, Yongxin 1 ; Zhao, Yan 7 ; Wang, Yuxiang 5 ; Li, Jun 6 ; Nan, Lili 2 ; Pang, Yongzhen 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
2.Gansu Agr Univ, Minist Educ, Coll Pratacultural Sci, Key Lab Grassland Ecosyst, Lanzhou 730070, Peoples R China
3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
4.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China
5.Xinjiang Agr Univ, Coll Grassland Sci, Key Lab Grassland Resources & Ecol Western Arid Re, Urumqi 833400, Peoples R China
6.Inner Mongolia Univ, Minist Educ, Sch Life Sci, Key Lab Herbage & Endem Crop Biol, Hohhot 010021, Peoples R China
7.Inner Mongolia Agr Univ, Minist Educ, Key Lab Grassland Resources, Coll Grassland Resources & Environm, Hohhot 010018, Peoples R China
关键词： MtAHA5; AHA10; Proanthocyanidins; H plus -ATPase; Medicago truncatula
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 294 卷
页码：
收录情况： SCI
摘要： Proanthocyanidins (PAs) are the second most abundant plant phenolic natural products. The proton membrane H+-ATPase (AHA) is required for PA transportation in vacuoles, but it remains unclear which AHA gene(s) encode tonoplast proton pump in M. truncatula. Here, we identified three Tnt1 mutant lines of MtAHA5, resulting in PAs deficit in seeds. MtAHA5 was preferentially expressed in developing seeds, exhibiting its highest transcript levels at early stages. Although MtAHA3, MtAHA4, and MtAHA9 shared similar transcript patterns with MtAHA5 and other structural genes involved in PA biosynthesis, their mutant lines did not exhibit any PA-deficit phenotypes. Subcellular localization analysis demonstrated that MtAHA5 is targeted to the tonoplast in tobacco leaves; conversely, MtAHA3 and MtAHA9 are localized to the cytoplasm, suggesting that MtAHA5 acts as a tonoplast proton pump but not MtAHA3 or MtAHA9. Further genetic analyses revealed that MtAHA5 could complement the PA-deficit phenotype in mtaha5 mutants and ataha10 mutants. Transient transcription assays indicated that MtAHA5 is activated by the MBW complex to regulate the PA accumulation. Collectively, our findings suggest that MtAHA5 serves as a tonoplast proton pump to generate the driving force for MATE1mediated transport of PA precursors into vacuoles.
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