文献类型： 外文期刊
作者： Li, Meng 1 ; Chen, Chunhai 2 ; Wang, Haigang 1 ; Qin, Huibin 1 ; Hou, Sen 1 ; Yang, Xukui 2 ; Jian, Jianbo 2 ; Gao, Peng 3 ; Liu, Minxuan 4 ; Mu, Zhixin 1 ;
作者机构： 1.Shanxi Agr Univ, Ctr Agr Genet Resources Res, Key Lab Crop Gene Resources & Germplasm Enhancemen, Minist Agr & Rural Affairs, Taiyuan 030031, Peoples R China
2.BGI Genom, Shenzhen 518083, Peoples R China
3.BGI, Shenzhen 518083, Peoples R China
4.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
期刊名称：SCIENTIFIC DATA （ 影响因子：5.8； 五年影响因子：8.9 ）
ISSN：
年卷期： 2024 年 11 卷 1 期
页码：
收录情况： SCI
摘要： "Cuohu Bazi" (CHBZ) is an ancient sorghum variety collected from the fields of China, known for its agronomic traits like dwarf stature, early maturation. In this study, we present the first telomere-to-telomere (T2T) and gap-free genome assembly of CHBZ using PacBio HiFi reads, Oxford Nanopore Technologies, and Hi-C data. The assembled genome comprises 724.85 Mb, effectively resolving all 3,913 gaps that were present in the previous sorghum BTx623 reference genome. Notably, the T2T assembly captures 10 centromeres and all 20 telomeres, providing strong support for their integrity. This assembly is of high quality in terms of contiguity (contig N50: 71.1 Mb), completeness (BUSCO score: 99.01%, k-mer completeness: 98.88%), and correctness (QV: 61.60). Repetitive sequences accounted for 70.41% of the genome and a total of 32,855 protein-coding genes have been annotated. Furthermore, 161 CHBZ-specific presence/absence variants genes have been identified when comparing to BTx623 genome. This study provides valuable insights for future research on sorghum genetics, genomics, and evolutionary history.
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