Identification and subcellular localization of NbIAP in the microsporidian Nosema bombycis
文献类型: 外文期刊
作者: Zhu, Feng 1 ; Xiao, Shengyan 3 ; Qin, Xingrong 1 ; Liu, Qunyan 1 ; Li, Hui 1 ; Ming, Dongfeng 1 ; Bai, Xingrong 2 ;
作者机构: 1.Zaozhuang Univ, Coll Life Sci, Zaozhuang 277160, Shandong, Peoples R China
2.Yunnan Acad Agr Sci, Inst Sericulture & Apiculture, Mengzi 661101, Yunnan, Peoples R China
3.Zaozhuang Univ, Coll Food Sci & Pharmaceut Engn, Zaozhuang 277160, Shandong, Peoples R China
关键词: Nosema bombycis; Inhibitor of apoptosis protein; Immunolocalization; RNAi
期刊名称:JOURNAL OF INVERTEBRATE PATHOLOGY ( 影响因子:2.795; 五年影响因子:2.802 )
ISSN: 0022-2011
年卷期: 2022 年 195 卷
页码:
收录情况: SCI
摘要: Many organisms go through a process of programmed cell death called apoptosis while newer cells are created. This has the effect of protecting the organism from cellular parasites and is a major line of defense against invading organisms. Apoptosis inhibitors, then, play an important role in aiding infectious agents by inhibiting caspase protease and thus the apoptopic pathway. In this study, we identified an inhibitor of apoptosis protein (IAP) in the microsporidian Nosema bombycis (NbIAP). NbIAP a composed of 218 amino acids containing two overlapping domains; the BIR domain and a zf-C3HC domain. We show, through indirect immunofluorescence, that NbIAP is present throughout the life cycle of N. bombycis and is localized in the nucleus of the parasite and therefor does not act on caspase protease directly. qRT-PCR analysis shows that the expression of the NbIAP gene was the highest on the first day of infection, then decreased to a relatively stable level. In addition, we show that the downregulation of the NbIAP gene directly inhibits the proliferation of N. bombycis. These findings suggest that NbIAP plays an important role in the N. bombycis life cycle.
- 相关文献
作者其他论文 更多>>
-
The complete mitochondrial genome of Pseudofabraea citricarpa (Dermateaceae: Helotiales) causing Citrus target spot
作者:Li, Hui;Xu, Songlin;Yang, Mei;Zhang, Wenjing;Duan, Zhengang;Chen, Quan;Li, Hui;Xu, Songlin;Yang, Mei;Zhang, Wenjing;Duan, Zhengang;Chen, Quan;Li, Hui;Ding, Mingliang;He, Jinhui;Ding, Mingliang
关键词:Mitochondrial genome; plant pathogen; phylogenetic analysis
-
Integration of metabolome, histochemistry and transcriptome analysis provides insights into lignin accumulation in oleocellosis-damaged flavedo of citrus fruit
作者:Zhou, Xianyan;Yang, Hongbin;Zhu, Feng;Xu, Rangwei;Deng, Xiuxin;Cheng, Yunjiang;Zhou, Xianyan;Yue, Jianqiang;Zhu, Chunhua;Li, Jinxue;Gao, Junyan;Zhou, Dongguo;Zhou, Xianyan;Yang, Hongbin;Zhu, Feng;Xu, Rangwei;Deng, Xiuxin;Cheng, Yunjiang
关键词:Green lemon; Oleocellosis disorder; Primary metabolites; Secondary metabolites; Lignification; Pathogen resistance
-
Identification of BmELP as an interaction partner of Bmtutl-519 in the silkworm, Bombyx mori
作者:Zhu, Feng;Qiao, Keyuan;Kang, Meiling;Ming, Dongfeng;Zhu, Feng;Gao, Jianhua;Xiao, Shengyan;Bai, Xingrong;Xiao, Shengyan
关键词:Bombyx mori; Nosema bombycis; Bmtutl-519; BmELP
-
First Report on Megaselia scalaris Loew (Diptera: Phoridae) Infestation of the Invasive Pest Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) in China
作者:Tang, Yunlin;Li, Qingyan;Xiang, Li;Gu, Ruocheng;Wu, Yanyan;Li, Tian;Wei, Junhong;Pan, Guoqing;Zhou, Zeyang;Tang, Yunlin;Li, Qingyan;Gu, Ruocheng;Wu, Yanyan;Li, Tian;Wei, Junhong;Pan, Guoqing;Zhou, Zeyang;Tang, Yunlin;Li, Qingyan;Gu, Ruocheng;Wu, Yanyan;Li, Tian;Wei, Junhong;Pan, Guoqing;Zhou, Zeyang;Xiang, Li;Zhang, Yonghong;Bai, Xingrong;Niu, Xiaohui;Zhou, Zeyang
关键词:Spodoptera frugiperda; invasive pest; natural enemies; Megaselia scalaris; pest management
-
CCT8 colocalizes with actin and beta-tubulin: Insight into its involvement in the cytoskeleton formation of the intracellular parasite Nosema bombycis
作者:Qi, Jingru;Chen, Ying;He, Ping;Shang, Ruisha;Sun, Fuzhen;Wang, Qiang;Zhang, Yiling;Tang, Xudong;Shen, Zhongyuan;Zhu, Feng;Shao, Li;Li, Jicheng;Wang, Qiang;Zhang, Yiling;Tang, Xudong;Shen, Zhongyuan;Zhu, Feng
关键词:Nosema bombycis; Chaperonin; CCT; Immunolocalization; RNAi
-
Identification and subcellular localisation of hexokinase-2 in Nosema bombycis
作者:Sun, Jiancheng;Chen, Hongli;Yao, Mingshuai;Zhu, Guanyu;Zhang, Yiling;Wang, Qiang;Shen, Zhongyuan;Zhu, Feng;Zhang, Yiling;Wang, Qiang;Shen, Zhongyuan;Zhu, Feng
关键词:microsporidia; silkworm; glucose metabolism; NbHXK2
-
Molecular epidemiology of Bombyx mori nucleopolyhedrovirus in Yunnan sericulture region, China
作者:Zhang, Yonghong;Tang, Fenfen;Shao, Yulan;Zhu, Feng;Bai, Xingrong
关键词:Bombyx mori nucleopolyhedrovirus; bro-d; evolutionary analysis; BmNPV