Identification of UDP-glucosyltransferase involved in the biosynthesis of phloridzin in Gossypium hirsutum
文献类型： 外文期刊
作者： Zhang, Xiaomeng 1 ; Tian, Xinquan 1 ; Luo, Junyu 1 ; Wang, Xiaoyang 1 ; He, Shoupu 1 ; Sun, Gaofei 2 ; Dong, Ruidan 3 ; Dai, Panhong 2 ; Wang, Xiao 5 ; Pan, Zhaoe 1 ; Chen, Baojun 1 ; Hu, Daowu 1 ; Wang, Liru 1 ; Pang, Baoyin 1 ; Xing, Aishuang 1 ; Fu, Guoyong 1 ; Wang, Baoquan 1 ; Cui, Jinjie 1 ; Ma, Lei 1 ; Du, Xiongming 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biobreeding & Integrated Util, Anyang 455000, Peoples R China
2.Anyang Inst Technol, Anyang 455000, Henan, Peoples R China
3.Zhengzhou Univ, State Key Lab Cotton Biol, Zhengzhou Res Base, Zhengzhou 450001, Peoples R China
4.Chinese Acad Agr Sci, Natl Nanfan Res Inst Sanya, Sanya 572024, Hainan, Peoples R China
5.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Henan Joint Int Lab Crop Multiom Res, Kaifeng 475004, Peoples R China
关键词： phloridzin; GWAS; molecular docking; population transcriptome analysis; overexpression
期刊名称：PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：7.0 ）
ISSN： 0960-7412
年卷期： 2025 年 121 卷 3 期
页码：
收录情况： SCI
摘要： Phloridzin has various functions, including antioxidant properties and the treatment of diabetes, and has long been used in pharmaceutical and physiological research. The glycosylation of phloretin is a key step in the biosynthesis of phloridzin. In this study, a genome-wide association study (GWAS) based on phloridzin content was applied, and the key gene GhUGT88F3 for phloridzin-specific biosynthesis was identified in cotton. A single-base deletion in GhUGT88F3 in haplotype I caused a frameshift mutation, leading to premature translation termination and a significant reduction in phloridzin content. Molecular docking revealed important amino acid residues for GhUGT88F3's UDP-glucose transfer activity. Additionally, the transcription factor GhMYB330 was found to positively regulate GhUGT88F3 expression through population transcriptome analysis and LUC experiment. Moreover, phloridzin content was significantly elevated in both GhUGT88F3 and GhMYB330 overexpression transgenic plants. This study expands the diversity of UDP-glucosyltransferases in plants and offers a potential strategy for the sustainable production of bioactive compounds with therapeutic potential.
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