PRMT5 Facilitates Infectious Bursal Disease Virus Replication through Arginine Methylation of VP1
文献类型: 外文期刊
作者: Hu, Xifeng 1 ; Chen, Zheng 1 ; Wu, Xiangdong 1 ; Fu, Qiuling 3 ; Chen, Zhen 3 ; Huang, Yu 3 ; Wu, Huansheng 1 ;
作者机构: 1.Jiangxi Agr Univ, Coll Anim Sci & Technol, Dept Vet Prevent Med, Nanchang, Peoples R China
2.Jiangxi Agr Univ, Coll Anim Sci & Technol, Jiangxi Prov Key Lab Anim Hlth, Nanchang, Peoples R China
3.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou, Peoples R China
关键词: IBDV VP1; arginine methylation; R426; PRMT5; polymerase activity; virology; post-translation modification; veterinary microbiology
期刊名称:JOURNAL OF VIROLOGY ( 影响因子:5.4; 五年影响因子:4.9 )
ISSN: 0022-538X
年卷期: 2023 年 97 卷 3 期
页码:
收录情况: SCI
摘要: The infectious bursal diseases virus (IBDV) polymerase, VP1 protein, is responsible for transcription, initial translation and viral genomic replication. Knowledge about the new kind of post-translational modification of VP1 supports identification of novel drugs against the virus. Because the arginine residue is known to be methylated by protein arginine methyltransferase (PRMT) enzyme, we investigated whether IBDV VP1 is a substrate for known PRMTs. In this study, we show that VP1 is specifically associated with and methylated by PRMT5 at the arginine 426 (R426) residue. IBDV infection causes the accumulation of PRMT5 in the cytoplasm, which colocalizes with VP1 as a punctate structure. In addition, ectopic expression of PRMT5 significantly enhances the viral replication. In the presence of PMRT5, enzyme inhibitor and knockout of PRMT5 remarkably decreased viral replication. The polymerase activity of VP1 was severely damaged when R426 mutated to alanine, resulting in impaired viral replication. Our study reports a novel form of post-translational modification of VP1, which supports its polymerase function to facilitate the viral replication.IMPORTANCE Post-translational modification of infectious bursal disease virus (IBDV) VP1 is important for the regulation of its polymerase activity. Investigation of the significance of specific modification of VP1 can lead to better understanding of viral replication and can probably also help in identifying novel targets for antiviral compounds. Our work demonstrates the molecular mechanism of VP1 methylation mediated by PRMT5, which is critical for viral polymerase activity, as well as viral replication. Our study expands a novel insight into the function of arginine methylation of VP1, which might be useful for limiting the replication of IBDV. Post-translational modification of infectious bursal disease virus (IBDV) VP1 is important for the regulation of its polymerase activity. Investigation of the significance of specific modification of VP1 can lead to better understanding of viral replication and can probably also help in identifying novel targets for antiviral compounds.
- 相关文献
作者其他论文 更多>>
-
Testing a biocontrol agent consortium for suppression of tomato bacterial wilt through rhizosphere microecological regulation
作者:Zheng, Xuefang;Wang, Jieping;Chen, Meichun;Chen, Yanping;Chen, Zheng;Liu, Bo;Wang, Mingkuang
关键词:Bacterial wilt; Biocontrol control agent consortium; Microecological regulation; Ralstonia solanacearum; Rhizosphere
-
Phosphorylation of VP1 Mediated by CDK1-Cyclin B1 Facilitates Infectious Bursal Disease Virus Replication
作者:Hu, Xifeng;Chen, Zheng;Wu, Xiangdong;Ding, Zhen;Wu, Huansheng;Hu, Xifeng;Chen, Zheng;Wu, Xiangdong;Ding, Zhen;Wu, Huansheng;Huang, Yu;Fu, Qiuling;Chen, Zhen
关键词:IBDV VP1; CDK1-cyclin B1; phosphorylation; Ser7; polymerase activity; viral polymerase; host kinase; viral protein phosphorylation; virology; virus replication
-
Antifungal activities of Bacillus velezensis FJAT-52631 and its lipopeptides against anthracnose pathogen Colletotrichum acutatum
作者:Deng, Ying-Jie;Chen, Zheng;Ruan, Chuan-Qing;Xiao, Rong-Feng;Liu, Bo;Chen, Mei-Chun;Wang, Jie-Ping;Deng, Ying-Jie;Lian, Heng-Ping;Chen, Mei-Chun;Wang, Jie-Ping
关键词:antifungal activity; biocontrol; Colletotrichum acutatum; lipopeptides; loquat
-
Mutations at site 207 of influenza a virus NS1 protein switch its function in regulating RIG-I-like receptors mediated antiviral responses
作者:Wang, Xingbo;Lin, Lulu;Si, Wei;Yan, Yan;Dong, Weiren;Jin, Yulan;Zhou, Jiyong;Chen, Zhen;Si, Wei;Huang, Yu;Zhou, Jiyong;Zhou, Jiyong
关键词:IFI35; RIG -I -like receptors; Influenza a virus; NS1; RNA recognition; K48-linked ubiquitination
-
The identification and expression of an interleukin-21 receptor in large yellow croaker (Larimichthys crocea)
作者:Wu, Hanyu;Teng, Yan;Mu, Pengfei;Chen, Jingjie;Chen, Xinhua;Chen, Xinhua;Fu, Qiuling
关键词:Larimichthys crocea; IL-21R; IL-21; Immune response
-
Detection and Characterization of Goose Astrovirus Infections in Hatcheries and Commercial Goose Flocks
作者:Ren, Zhihao;Zhang, Qingshui;Li, Jinxin;Yu, Ziding;Su, Jingliang;Fu, Guanghua;Liu, Rongchang;Huang, Yu
关键词:
-
Fowl adenovirus serotype 4 52/55k protein triggers PKR degradation by ubiquitin-proteasome system to evade effective innate immunity
作者:He, Qing;Lu, Shaohua;Lin, Yun;Xu, Lihui;Wang, Quanxi;He, Qing;Lu, Shaohua;Lin, Yun;Xu, Lihui;Wang, Quanxi;Chen, Zhen;Wang, Quanxi
关键词:Fowl adenovirus serotype 4; 52; 55 K protein; Protein kinase R; Ubiquitin -proteasome system; Immune escape