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RNA N6-methyladenosine of DHAPAT and PAP involves in regulation of diapause of Bombyx mori via the lipid metabolism pathway

文献类型： 外文期刊

作者： Chen, Yan-Hua 1 ; Jiang, Tao 3 ; Yasen, Ayinuer 1 ; Fan, Bing-Yan 1 ; Zhu, Juan 1 ; Wang, Mei-Xian 1 ; Shen, Xing-Jia 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Coll Biotechnol, Jiangsu Key Lab Sericultural Biol & Biotechnol, Zhenjiang 212100, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Sericulture Res Inst, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Zhenjiang 212100, Jiangsu, Peoples R China

3.Silkworm Egg Inst Jiangsu Prov, Wuxi 214000, Jiangsu, Peoples R China

关键词： Bombyx mori; DHAPAT; diapause; lipid metabolism; PAP; post-transcriptional regulation; RNA N6-adenosine methylation; YTHDF3

期刊名称：BULLETIN OF ENTOMOLOGICAL RESEARCH （ 影响因子：1.9； 五年影响因子：1.9 ）

ISSN： 0007-4853

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Environment-induced epigenetics are involved in diapause regulation, but the molecular mechanism that epigenetically couples nutrient metabolism to diapause regulation remains unclear. In this study, we paid special attention to the significant differences in the level of N6-adenosine methylation (m(6)A) of dihydroxyacetone phosphate acyltransferase (DHAPAT) and phosphatidate phosphatase (PAP) genes in the lipid metabolism pathway of the bivoltine silkworm (Bombyx mori) strain Qiufeng developed from eggs incubated at a normal temperature (QFHT, diapause egg producer) compared to those from eggs incubated at a low temperature (QFLT, non-diapause egg producer). We knocked down DHAPAT in the pupal stage of the QFLT group, resulting in the non-diapause destined eggs becoming diapausing eggs. In the PAP knockdown group, the colour of the non-diapause destined eggs changed from light yellow to pink 3 days after oviposition, but they hatched as normal. Moreover, we validated that YTHDF3 binds to m(6)A-modified DHAPAT and PAP mRNAs to promote their stability and translation. These results suggest that RNA m(6)A methylation participates in the diapause regulation of silkworm by changing the expression levels of DHAPAT and PAP and reveal that m(6)A epigenetic modification can be combined with a lipid metabolism signal pathway to participate in the regulation of insect diapause traits, which provides a clearer image for exploring the physiological basis of insect diapause.

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