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An innovative immunochromatographic assay employing Pt-Pd bimetallic nanoparticles as labels for the detection of foot-and-mouth disease virus serotype O

文献类型： 外文期刊

作者： Sun, Yanyan 1 ; Li, Xin 1 ; Lin, Mi 1 ; Yang, Guang 1 ; He, Huali 1 ; Bao, Yanfang 1 ; Li, Fengsong 1 ; Jiang, Tao 1 ;

作者机构： 1.Lanzhou Shouyan Biotechnol Co Ltd, Lanzhou 730070, Peoples R China

2.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Anim Virol,Minist Agr, Lanzhou 730046, Peoples R China

3.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Xujaping 1, Lanzhou 730046, Peoples R China

关键词： Immunochromatographic assay; Pt-Pd bimetallic nanoparticles; Peroxide-like; Foot-and-mouth disease virus type O

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.0； 五年影响因子：2.9 ）

ISSN： 1570-0232

年卷期： 2024 年 1235 卷

页码：

收录情况： SCI

摘要： Objective: We created a novel, high sensitivity immunochromatographic assay that allows for clear and precise quantitative analysis by employing innovative bimetallic nanoparticles with peroxide-like activity as markers for the preparation of the test strip. Methods: Initially, we synthesized Pt-Pd bimetallic nanoparticles through the reduction of K2PtCl4 and Na2PdCl4 using ascorbic acid (AA) in an ultrasonic water bath. These bimetallic nanoparticles were then utilized to label purified antigens from the foot-and-mouth disease virus (FMDV) type O (FMDV-146S), resulting in the creation of antigen-captured nanomarkers. Upon completion of the antigen-antibody reaction, we introduced a color-developing agent (3,3',5,5'-tetramethylbenzidine) for cascade amplification, significantly enhancing detection sensitivity while ensuring clear and accurate quantitative analysis. Results: The quantitative detection sensitivity achieved was 1:2(8)/test, with a linear range spanning from 1:2(6) similar to 1:2(9) /test. For FMDV type O positive serum, the detection sensitivity reached 96.7 %. Furthermore, this method exhibited a 95 % detection sensitivity for FMDV negative serum, FMDV type A and type AsiaI positive sera, as well as sera positive for other common viral diseases in animals. In comparison to the OIE-recommended LPB-ELISA, this approach displayed higher correlation (correlation coefficient = 0.909). Innovation was at the core of establishing this immunochromatographic assay based on Pt-Pd bimetallic nanoparticles for the detection of FMDV antibodies. Conclusion: The findings revealed a striking 2(4)-fold improvement in sensitivity when compared to colloidal gold, accompanied by a strong correlation coefficient (R-2 > 0.9). This suggests a robust and consistent linear association in the results. This method represents a significant advancement in the field of rapid immunochromatographic assays, offering a promising alternative application for bimetallic nanoparticles.

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