您好，欢迎访问宁夏农林科学院 机构知识库！
宁夏农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genome-wide transcriptomic analysis identifies candidate genes involved in jasmonic acid-mediated salt tolerance of alfalfa

文献类型： 外文期刊

作者： Yang, Tianhui 1 ; Tian, Mei 2 ; Gao, Ting 1 ; Wang, Chuan 1 ; Wang, Xiaochun 1 ; Chen, Caijin 3 ; Yang, Weidi 1 ;

作者机构： 1.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan, Ningxia, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Inst Hort Sci, Yinchuan, Ningxia, Peoples R China

3.Ningxia Acad Agr & Forestry Sci, Branch Inst Guyuan, Guyuan, Ningxia, Peoples R China

关键词： Salt stress; Transcriptomic analysis; Alternative splicing; Alfalfa; Jasmonic acid

期刊名称：PEERJ （ 影响因子：2.7； 五年影响因子：3.1 ）

ISSN： 2167-8359

年卷期： 2023 年 11 卷

页码：

收录情况： SCI

摘要： Soil salinity imposes a major threat to plant growth and agricultural productivity. Despite being one of the most common fodder crops in saline locations, alfalfa is vulnerable to salt stress. Jasmonic acid (JA) is a phytohormone that influences plant response to abiotic stimuli such as salt stress. However, key genes and pathways by which JA-mediated salt tolerance of alfalfa are little known. A comprehensive transcriptome analysis was performed to elucidate the underlying molecular mechanisms of JA-mediated salt tolerance. The transcripts regulated by salt (S) compared to control (C) and JA+salt (JS) compared to C were investigated. Venn diagram and expression pattern of DEGs indicated that JS further altered a series of genes expression regulated by salt treatment, implying the roles of JA in priming salt tolerance. Enrichment analysis revealed that DEGs exclusively regulated by JS treatment belonged to primary or secondary metabolism, respiratory electron transport chain, and oxidative stress resistance. Alternatively, splicing (AS) was induced by salt alone or JA combined treatment, with skipped exon (SE) events predominately. DEGs undergo exon skipping involving some enriched items mentioned above and transcription factors. Finally, the gene expressions were validated using quantitative polymerase chain reaction (qPCR), which produced results that agreed with the sequencing results. Taken together, these findings suggest that JA modulates the expression of genes related to energy supply and antioxidant capacity at both the transcriptional and post-transcriptional levels, possibly through the involvement of transcription factors and AS events.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：宁夏农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台