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Stable isotope probing reveals compositional and functional shifts in active denitrifying communities along the soil profile in an intensive agricultural area

文献类型： 外文期刊

作者： Chen, Shuaimin 1 ; Kuramae, Eiko E. 2 ; Jia, Zhongjun 5 ; Liu, Binbin 1 ;

作者机构： 1.Chinese Acad Sci, Ctr Agr Resources Res, Inst Genet & Dev Biol, 286 Huaizhong Rd, Shijiazhuang 050021, Peoples R China

2.Jilin Acad Agr Sci, Inst Agr Resources & Environm, 1363 Shengtai St, Changchun 130033, Peoples R China

3.Netherlands Inst Ecol NIOO KNAW, Dept Microbial Ecol, NL-6708 PB Wageningen, Netherlands

4.Univ Utrecht, Inst Environm Biol, Ecol & Biodivers, Padualaan 8, NL-3584 CH Utrecht, Netherlands

5.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Wetland Ecol & Environm, State Key Lab Black Soils Conservat & Utilizat, Changchun 130102, Peoples R China

关键词： Nitrate leaching; Denitrification; Stable isotope probing; Vadose zone; Active denitrifiers

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：9.8； 五年影响因子：9.6 ）

ISSN： 0048-9697

年卷期： 2024 年 907 卷

页码：

收录情况： SCI

摘要： Denitrifying microbial communities in the vadose zone play an essential role in eliminating the nitrate leached from agricultural practices. This nitrate could otherwise contaminate groundwater and threaten public health. Here, we utilized stable isotope probing combined with amplicon sequencing and functional gene quantification to inspect the composition and function of heterotrophic denitrifying microorganisms along a 9-m soil profile in an intensive agricultural area. Dramatic differences in the composition of the active denitrifiers were uncovered between the surface soil and deep layers of the vadose zone. The main denitrifying bacterial taxa identified from 13C-DNA fractions were Pseudomonadaceae (Pseudomonas), Rhodocyclaceae (Azoarcus), and Burkholderiaceae in the surface soil (0-0.2 m), and were Pseudomonadaceae (Pseudomonas), Burkholderiaceae, Bacillaceae (Bacillus), and Paenibacillaceae (Ammoniphilus) in the deep layers (0.5-9.0 m). Analysis of the functional genes (nirS, nirK, and nosZ) in isotope-labeled DNA revealed an upward nos/nir ratio with increasing soil depth, which may account for the higher nitrous oxide emission potential in the surface soil, as compared to the deeper sand-rich, low organic carbon layers. This study improves our understanding of active denitrifying microbes in the vadose zone and helps in developing techniques to reduce nitrate pollution in groundwater.

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