A rapid and cost-effective approach for quantifying the content of genetically modified organism to facilitate the enforcement of labeling regulation
文献类型： 外文期刊
作者： Yao, Xiaoqing 1 ; Li, Jun 1 ; Wan, Danfeng 1 ; Gao, Hongfei 1 ; Li, Yunjing 1 ; Xiao, Fang 1 ; Zhai, Shanshan 1 ; Wu, Gang 1 ; Wu, Yuhua 1 ;
作者机构： 1.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr & Rural Affairs, Wuhan 430062, Peoples R China
关键词： Genetically modified organism; Labeling; Rapid quantification; 2-Delta Delta Ct method; T-test
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）
ISSN： 0026-265X
年卷期： 2025 年 214 卷
页码：
收录情况： SCI
摘要： The current accurate quantitative technology, such as standard curve-based quantitative real time PCR (qPCR) and digital PCR (dPCR), is time-consuming and expensive, resulting in high costs for the implementation of the GMO labeling policy. This study proposed a rapid quantitative strategy that combines the 2-Delta Delta Ctmethod with the t-test of Delta Ct values between the test samples and the reference control. This method allowed for an approximate estimation of the GMO content of test samples while identifying the GM events. GMO labeling or exempt for most samples can be determined based on the rapid estimation of GMO content and the t-test result of the Delta Ct values between the test samples and the reference control. This approach streamlines the quantitative analysis process, makes the quantification more accessible and affordable, and supports compliance with GMO labeling regulations worldwide.
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