Identification and functional characterization of Gh_D01G0514 (GhNAC072) transcription factor in response to drought stress tolerance in cotton
文献类型： 外文期刊
作者： Mehari, Teame Gereziher 1 ; Xu, Yanchao 1 ; Magwanga, Richard Odongo 2 ; Umer, Muhammad Jawad 1 ; Shiraku, Margaret Linyerera 1 ; Hou, Yuqing 1 ; Wang, Yuhong 1 ; Wang, Kunbo 1 ; Cai, Xiaoyan 1 ; Zhou, Zhongli 1 ; Liu, Fang 3 ;
作者机构： 1.Chinese Acad Agr Sci ICR, State Key Lab Cotton Biol, Inst Cotton Res, CAAS, Anyang 455000, Henan, Peoples R China
2.Jaramogi Oginga Odinga Univ Sci & Technol JOOUST, Sch Biol & Phys Sci SBPS, Main Campus,POB 210-40601, Bondo, Kenya
3.Zhengzhou Univ, Sch Agr Sci, Zhengzhou 450001, Henan, Peoples R China
关键词： Cotton; NAC; Virus induced gene silencing; Drought; Green fluorescence protein; Yeast two hybrid; Coexpression network analysis
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）
ISSN： 0981-9428
年卷期： 2021 年 166 卷
页码：
收录情况： SCI
摘要： Cotton encounters long-term drought stress problems resulting in major yield losses. Transcription factors (TFs) plays an important role in response to biotic and abiotic stresses. The coexpression patterns of gene networks associated with drought stress tolerance were investigated using transcriptome profiles. Applying a weighted gene coexpression network analysis, we discovered a salmon module with 144 genes strongly linked to drought stress tolerance. Based on coexpression and RT-qPCR analysis GH_D01G0514 was selected as the candidate gene, as it was also identified as a hub gene in both roots and leaves with a consistent expression in response to drought stress in both tissues. For validation of GH_D01G0514, Virus Induced Gene Silencing was performed and VIGS plants showed significantly higher excised leaf water loss and ion leakage, while lower relative water and chlorophyll contents as compared to WT (Wild type) and positive control plants. Furthermore, the WT and positive control seedlings showed higher CAT and SOD activities, and lower activities of hydrogen peroxide and MDA enzymes as compared to the VIGS plants. Gh_D01G0514 (GhNAC072) was localized in the nucleus and cytoplasm. Y2H assay demonstrates that Gh_D01G0514 has a potential of auto activation. It was observed that the Gh_D01G0514 was highly upregulated in both tissues based on RNA Seq and RT-qPCR analysis. Thus, we inferred that, this candidate gene might be responsible for drought stress tolerance in cotton. This finding adds significantly to the existing knowledge of drought stress tolerance in cotton and deep molecular analysis are required to understand the molecular mechanisms underlying drought stress tolerance in cotton.
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