Selenium Treatment Ameliorates the Adverse Effects Caused by Dynamin Gene Knockdown in Bombyx mori
文献类型： 外文期刊
作者： Hong, Ting-Ting 1 ; Hu, Shuo 2 ; Hu, Fei 1 ; Ge, Wen-Jie 1 ; Thakur, Kiran 1 ; Tang, Shun-Ming 3 ; Wei, Zhao-Jun 1 ;
作者机构： 1.Hefei Univ Technol, Sch Food & Biol Engn, Hefei, Peoples R China
2.North Minzu Univ, Sch Biol Sci & Engn, Yinchuan, Peoples R China
3.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural Biol & Biotechnol, Zhenjiang, Peoples R China
4.Chinese Acad Agr Sci, Sericultural Res Inst, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr, Zhenjiang, Peoples R China
关键词： Bombyx mori; dynamin; gene expression; Se; siRNAs
期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.9； 五年影响因子：2.2 ）
ISSN： 0739-4462
年卷期： 2024 年 117 卷 1 期
页码：
收录情况： SCI
摘要：
Our previous research reported the influence of 50 mu M selenium (Se) on the cytosolization (endocytosis) pathway, which in turn stimulates the growth and development of Bombyx mori. Lately, dynamin is recognized as one of the key proteins in endocytosis. To explore the underlying mechanisms of Se impact, the dynamin gene was knocked down by injecting siRNAs (Dynamin-1, Dynamin-2, and Dynamin-3). This was followed by an analysis of the target gene and levels of silk protein genes, as well as growth and developmental indices, Se-enrichment capacity, degree of oxidative damage, and antioxidant capacity of B. mori. Our findings showed a considerable decrease in the relative expression of the dynamin gene in all tissues 24 h after the interference and a dramatic decrease in the silkworm body after 48 h. RNAi dynamin gene decreased the silkworm body weight, cocoon shell weight, and the ratio of cocoon. In the meantime, malondialdehyde level increased and glutathione level and superoxide dismutase/catalase activities decreased. 50 mu M Se markedly ameliorated these growth and physiological deficits as well as decreases in dynamin gene expression. On the other hand, there were no significant effects on fertility (including produced eggs and laid eggs) between the interference and Se treatments. Additionally, the Se content in the B. mori increased after the dynamin gene interference. The dynamin gene was highly expressed in the silk gland and declined significantly after interference. Among the three siRNAs (Dynamin-1, Dynamin-2, and Dynamin-3), the dynamin-2 displayed the highest interference effects to target gene expression. Our results demonstrated that 50 mu M Se was effective to prevent any adverse effects caused by dynamin knockdown in silkworms. This provides practical implications for B. mori breeding industry.
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