Comparative alternative polyadenylation profiles in differentiated adipocytes of subcutaneous and intramuscular fat tissue in cattle
文献类型： 外文期刊
作者： Meng, Xiangge 1 ; Li, Chengping 2 ; Hei, Yu 1 ; Zhou, Xiang 1 ; Zhou, Guoli 2 ;
作者机构： 1.Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Key Lab Swine Genet & Breeding, Minist Agr,Minist Educ,Coll Anim Sci & Technol, Wuhan, Peoples R China
2.Liaocheng Univ, Coll Life Sci, Liaocheng, Peoples R China
3.Hubei Hongshan Lab, Wuhan, Peoples R China
4.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Shenzhen, Peoples R China
5.Chinese Acad Agr Sci, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab, Minist Agr,Agr Genom Inst Shenzhen, Shenzhen, Peoples R China
关键词： Alternative polyadenylation; Intramuscular adipocytes; Subcutaneous adipocytes; Differentiation; Cattle
期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）
ISSN： 0378-1119
年卷期： 2024 年 894 卷
页码：
收录情况： SCI
摘要： Alternative polyadenylation (APA) is a key molecular mechanism involved in the post-transcriptional regulation of gene expression, which has been proven to play a critical role in cell differentiation. In the present study, we performed IVT-SAPAS sequencing to profile the dynamic changes of APA sites in bovine subcutaneous preadipocytes and intramuscular preadipocytes during adipogenesis. A total of 52621 high quality APA sites were identified in preadipocytes and adipocytes. Compared with preadipocytes, the increased usage of canonical AATAAA was observed in the cell-biased APA sites of adipocytes. Furthermore, 1933 and 2140 differentially expressed APA (DE-APA) sites, as well as 341 and 337 untranslated region-APA (UTR-APA) switching genes were identified in subcutaneous preadipocytes and intramuscular preadipocytes during adipogenesis, respectively. The UTR-APA switching genes showed divergent trends in preadipocytes, among which UTR-APA switching genes in intramuscular preadipocytes tended to use shorter 3 ' UTR for differentiation into mature adipocytes. APA events mediated by UTR-APA switching in intramuscular adipocytes were enriched in lipid synthesis and adipocyte differentiation. TRIB3, WWTR1, and INSIG1 played important roles in the differentiation of intra-muscular preadipocytes. Briefly, our results provided new insights into understanding the mechanisms of bovine adipocyte differentiation.
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