Pseudorabies Virus Regulates the Extracellular Translocation of Annexin A2 To Promote Its Proliferation
文献类型: 外文期刊
作者: Weng, Maoyang 1 ; Guo, Zhenhua 2 ; Lu, Qingxia 2 ; Jin, Qianyue 2 ; Jiang, Yao 2 ; Wang, Fangyu 2 ; Guo, Junqing 2 ; Xing, Guangxu 2 ; Qiao, Songlin 2 ; Zhang, Gaiping 1 ;
作者机构: 1.Northwest A&F Univ, Coll Vet Med, Yangling, Shaanxi, Peoples R China
2.Henan Acad Agr Sci, Key Lab Anim Immunol Minist Agr, Henan Prov Key Lab Anim Immunol, Zhengzhou, Henan, Peoples R China
3.Peking Univ, Sch Adv Agr Sci, Beijing, Peoples R China
4.Longhu Modern Immun Lab, Zhengzhou, Henan, Peoples R China
5.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China
关键词: pseudorabies virus; US3; annexin A2; Src; phosphorylation; antiviral; antiviral agents; protein phosphorylation
期刊名称:JOURNAL OF VIROLOGY ( 影响因子:5.4; 五年影响因子:4.9 )
ISSN: 0022-538X
年卷期: 2023 年 97 卷 3 期
页码:
收录情况: SCI
摘要: PRV belongs to the alphaherpesvirus and has recently re-emerged in China, causing severe economic losses. Recent studies also indicate that PRV may pose a potential public health challenge. Pseudorabies virus (PRV) infection causes enormous economic losses to the pork industry and severe health consequences in many hosts. Annexin A2 (ANXA2) is a membrane-associated protein with various intracellular functions associated with many viral infections. However, the role of ANXA2 in alphaherpesvirus replication is still not explored. In the present study, we identified the interaction between ANXA2 and PRV US3. The deficiency of ANXA2 significantly restricted PRV proliferation. PRV infection or US3 overexpression led to ANXA2 extracellular translocation. Furthermore, we confirmed that PRV or US3 could lead to the phosphorylation of the Tyr23 ANXA2 and Tyr419 Src kinase, which was associated with the ANXA2 cell surface transposition. US3 can also bind to Src in an ANXA2-independent manner and enhance the interaction between Src and ANXA2. Additionally, inhibitors targeting ANXA2 (A2ti-1) or Src (PP2) could remarkably inhibit PRV propagation in vitro and protect mice from PRV infection in vivo. Collectively, our findings broaden our understanding of the molecular mechanisms of ANXA2 in alphaherpesvirus pathogenicity and suggest that ANXA2 is a potential therapeutic target for treating alphaherpesvirus-induced infectious diseases.IMPORTANCE PRV belongs to the alphaherpesvirus and has recently re-emerged in China, causing severe economic losses. Recent studies also indicate that PRV may pose a potential public health challenge. ANXA2 is a multifunctional calcium- and lipid-binding protein implicated in immune function, multiple human diseases, and viral infection. Herein, we found that ANXA2 was essential to PRV efficient proliferation. PRV infection resulted in the extracellular translocation of ANXA2 through phosphorylation of ANXA2 and Src. ANXA2 and Src formed a complex with PRV US3. Importantly, inhibitors targeting ANXA2 or Src prevented PRV infection in vitro and in vivo. Therefore, our studies reveal a novel strategy by which alphaherpesvirus modifies ANXA2 to promote its replication and highlight ANXA2 as a target in developing novel promising antivirus agents in viral therapy.
- 相关文献
作者其他论文 更多>>
-
Infection phase-dependent dynamics of the viral and host N6-methyladenosine epitranscriptome in the lifecycle of an oncogenic virus in vivo
作者:Zhuang, Guoqing;Zhao, Xuyang;Jin, Jiaxin;Zhu, Xiaojing;Wang, Rui;Zhai, Yunyun;Lu, Wenlong;Sun, Aijun;Zhang, Gaiping;Zhuang, Guoqing;Zhao, Xuyang;Jin, Jiaxin;Zhu, Xiaojing;Wang, Rui;Zhai, Yunyun;Lu, Wenlong;Sun, Aijun;Zhang, Gaiping;Liao, Yifei;Teng, Man;Luo, Jun;Zhang, Gaiping;Teng, Man;Luo, Jun;Zhang, Gaiping;Teng, Man;Yao, Yongxiu;Nair, Venugopal;Yao, Yongxiu;Nair, Venugopal;Yao, Wen;Zhang, Gaiping
关键词:epitranscriptome; N6-methyladenosine (m(6)A); oncogenic viruses; tumorigenesis
-
Spy&IAC enables specific capture of SpyTagged proteins for rapid assembly of plug-and-display nanoparticle vaccines
作者:Chen, Yilan;Ren, Dongna;Zhang, Ning;Zhang, Gaiping;Chen, Yilan;Li, Minghui;Liu, Siyuan;Chang, Zejie;Ren, Dongna;Li, Ruiqi;Zhang, Ning;Sun, Xueke;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Li, Minghui;Liu, Siyuan;Chang, Zejie;Sun, Xueke;Zhang, Gaiping;Zhang, Gaiping
关键词:SpyTag; Immunoaffinity chromatography; Modular assembly; Nanoparticles; Coronavirus
-
Establishment and characterization of a novel indirect ELISA method based on ASFV antigenic epitope-associated recombinant protein
作者:Jin, Jiaxin;Zhang, Yuanyuan;Lu, Wenlong;Zhang, Shuai;Zhao, Xuyang;Wu, Yanan;Zhang, Angke;Zhang, Gaiping;Sun, Aijun;Zhuang, Guoqing;Jin, Jiaxin;Zhang, Yuanyuan;Lu, Wenlong;Zhang, Shuai;Zhao, Xuyang;Wu, Yanan;Zhang, Angke;Zhang, Gaiping;Sun, Aijun;Zhuang, Guoqing;Bai, Yilin;Sun, Yaning;Sun, Yaning;Zhang, Gaiping
关键词:African swine fever; African swine fever virus; Antigenic epitope; Recombinant protein; Indirect ELISA
-
Unamplified system for sensitive and typing detection of ASFV by the cascade platform that CRISPR-Cas12a combined with graphene field-effect transistor
作者:Wang, Hua;Zhou, Yuan;Liu, Yujie;Chen, Lei;Sun, Yang;Li, Zhengping;Chen, Shuo;Yang, Cheng;Sun, Wenbo;Zhang, Zidong;Guo, Junqing
关键词:ASFV; Graphene; Field-effect transistor; CRISPR/Cas12a; Typing; Amplification free detection
-
HSPA5 Promotes Attachment and Internalization of Porcine Epidemic Diarrhea Virus through Interaction with the Spike Protein and the Endo-/Lysosomal Pathway
作者:Zhou, Chuanjie;Cheng, Anchun;Zhang, Gaiping;Zhou, Chuanjie;Liu, Yunchao;Wei, Qiang;Yang, Suzhen;Zhang, Gaiping;Chen, Yumei;Zhang, Gaiping;Zhang, Gaiping
关键词:PEDV; HSPA5; attachment; internalization; endo-; lysosome pathway
-
Development and Evaluation of a Blocking Lateral Flow Assay Strip for Detection of Newcastle Disease Virus Antibodies
作者:Lv, Rongzhou;Zhang, Yuhang;Wang, Xun;Meng, Zekun;Zhang, Gaiping;Guo, Junqing;Li, Ge;Wang, Li;Chai, Shujun;Li, Qingmei;Zhang, Gaiping;Zhang, Gaiping
关键词:Newcastle disease virus; blocking lateral flow bLFA strip; neutralizing antibodies; HI; monoclonal antibodies
-
Virtual Screening-Based Peptides Targeting Spike Protein to Inhibit Porcine Epidemic Diarrhea Virus (PEDV) Infection
作者:Xu, Qian;Zhang, Mengting;Zhang, Gaiping;Xu, Qian;Wang, Fangyu;Jiao, Wenqiang;Zhang, Mengting;Xing, Guangxu;Feng, Hua;Sun, Xuefeng;Hu, Man;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词:porcine epidemic diarrhea virus; S1 C-terminal domain (CTD) protein; virtual screening; SPR; antiviral peptides; qRT-PCR; indirect immunofluorescence