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ZF protein C2H2-71 regulates the soluble solids content in tomato by inhibiting LIN5

文献类型： 外文期刊

作者： Li, Fangman 1 ; Lin, Junshen 1 ; Ahiakpa, John Kojo 1 ; Gai, Wenxian 1 ; Tao, Jinbao 1 ; Ge, Pingfei 1 ; Zhang, Xingyu 1 ; Mu, Yizhuo 1 ; Ye, Jie 1 ; Zhang, Yuyang 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Germplasm Innovat Utilizat Hort Crops, Wuhan 430070, Peoples R China

2.Hubei Hongshan Lab, Wuhan 430070, Peoples R China

3.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Wuhan 430070, Peoples R China

4.Chinese Acad Agr Sci, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Minist Agr & Rural Affairs,Agr Gen, Shenzhen 518000, Peoples R China

5.Univ Calif Davis, Coll Agr & Environm Sci, Davis, CA 95616 USA

关键词： Solanum lycopersicum L.; transcription factor; fruit flavor; cell wall invertase; LIN5

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.4； 五年影响因子：4.8 ）

ISSN： 2095-3119

年卷期： 2025 年 24 卷 6 期

页码：

收录情况： SCI

摘要： Soluble solids content (SSC) plays an important role in determining the flavor of tomato fruits. Tomato fruit SSC is transcriptionally regulated via sugar metabolism. Previous studies have predominantly focused on the role of C2H2-type zinc finger proteins in tomato growth and development. However, the specific regulatory mechanisms of C2H2 in the accumulation of soluble solids in tomato fruits are not fully understood. This study used eight tomato accessions with varying levels of SSC to study the expression of SlC2H2 family genes in red ripe fruits. The levels of SlC2H2-71 expression were found to be significantly reduced in high-SSC accessions compared to low-SSC accessions. Several Slc2h2-71 mutant lines were developed using the CRISPR-Cas9 system, which led to elevated levels of soluble solids, fructose, glucose, malic acid, and citric acid in mature red ripe fruits. However, the sucrose content in the edited Slc2h2-71 mutant lines was generally lower. RNA-seq analysis revealed that fruits from the mutant lines had altered expression of genes related to the sugar and acid metabolic pathways, which was further confirmed by quantitative real-time PCR. Specifically, the expression of SlLIN5 encoding the cell wall invertase (CWIN) was elevated. The yeast one-hybrid (Y1H) assay, 35S::UAS-GUS, dual-luciferase reporter systems and electrophoretic mobility shift assay (EMSA) demonstrated that C2H2-71 regulates tomato sugar metabolism by directly binding to the promoter region of SlLIN5, culminating in the repression of its transcriptional activity. The activity of acid invertase exhibited a significantly higher level in the SlC2H2-71 knock-out lines compared to the control lines. In summary, the regulation of tomato fruit SSC by C2H2-71 involves the inhibition of SlLIN5 expression.

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