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Genome-wide identification of the ECERIFERUM (CER) gene family in cabbage and critical role of BoCER4.1 in wax biosynthesis

文献类型： 外文期刊

作者： Cao, Wenxue 1 ; Sun, Huagang 3 ; Wang, Caihong 2 ; Yang, Limei 2 ; Zhang, Yangyong 2 ; Zhuang, Mu 2 ; Lv, Honghao 2 ; Wang, Yong 2 ; Liu, Feng 1 ; Ji, Jialei 2 ;

作者机构： 1.Hunan Agr Univ, Engn Res Ctr Educ, Minist Germplasm Innovat & Breeding New Varieties, Key Lab Vegetable Biol Hunan Prov,Coll Hort, Changsha 410128, Peoples R China

2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

3.China Vegetable Seed Technol Co Ltd Chongqing, Chongqing 402561, Peoples R China

关键词： Cabbage; CER gene family; Cuticular wax synthesis; Drought resistance; BoCER4

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2025 年 222 卷

页码：

收录情况： SCI

摘要： The ECERIFERUM (CER) gene family is essential for the biosynthesis of plant cuticular wax. In this study, 32 BoCER genes were identified in cabbage through genome-wide analysis. We found that the BoCER genes are highly conserved with their homologous counterparts in Arabidopsis thaliana. However, there was a significant divergence in the expression pattern among the BoCER paralogs, which suggests the occurrence of functional specialization during evolution. The expression analysis also showed that most of the BoCER genes are expressed in the aboveground part. Cis-regulatory element analysis suggested that BoCER genes could potentially be regulated through coordinated light and hormonal signaling. Furthermore, the abscisic acid and drought treatments markedly upregulated multiple BoCER genes, highlighting their involvement in abiotic stress responses. The functional analysis using CRISPR/Cas9-mediated knockout showed that BoCER4.1 governs the biosynthesis of alcohol. In situ hybridization localized the expression of BoCER4.1 to the tapetum, microspores, stem epidermis, and vascular bundles, while subcellular localization assays indicated its location in the endoplasmic reticulum, which aligns it with the biosynthetic machinery for wax. A phenotypic analysis revealed that the cuticles of the BoCER4.1 mutants were more permeable, and this was characterized by accelerated water loss and chlorophyll leaching. Correspondingly, the drought resistance of cababge with BoCER4.1 knockout was significantly reduced, accompanied by increased malondialdehyde content and decreased proline content under drought condition. This study provides new insights into the role of BoCERs in wax biosynthesis of cabbage, and also provides scientific basis for genetic improvement of drought resistance in cabbage.

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