Nuclear receptor hormone receptor 39 is required for locust moulting by regulating the chitinase and carboxypeptidase genes
文献类型： 外文期刊
作者： Zhao, X. -M. 3 ; Qin, Z. -Y. 1 ; Zhang, J. 1 ; Yang, Y. 1 ; Jia, P. 1 ; Yang, Q. 3 ; Ma, E. -B. 1 ; Zhang, J. -Z. 1 ;
作者机构： 1.Shanxi Univ, Res Inst Appl Biol, Taiyuan 030006, Shanxi, Peoples R China
2.Shanxi Univ, Coll Life Sci, Taiyuan, Shanxi, Peoples R China
3.Chinese Acad Agr Sci, Inst Plant Protect, Beijing, Peoples R China
关键词： nuclear receptor; LmHR39; carboxypeptidase; chitinase; Locusta migratoria
期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）
ISSN： 0962-1075
年卷期： 2019 年 28 卷 4 期
页码：
收录情况： SCI
摘要： The nuclear receptor-mediated 20-hydroxyecdysone (20E) signalling pathway plays crucial roles in insects by initiating and regulating moulting and metamorphosis. In the present study, we identified and characterized a cDNA encoding a putative nuclear receptor protein (Locusta migratoria hormone receptor 39, LmHR39) based on L. migratoria transcriptomics data. Reverse-transcription quantitative PCR (RT-qPCR) revealed that LmHR39 shows low-level expression in the early days of fifth-instar nymphs, and peak expression occurs on day 5, which is followed by a decrease before ecdysis. LmHR39 transcription could be induced by 20E in vivo and was significantly suppressed by knocking down the expression of the L. migratoria ecdysone receptor gene and early-late gene LmHR3. After RNA interference of LmHR39 with double-stranded RNA (dsRNA), 85% of the insects showed abnormal morphology, with curly wings after moulting and delayed eclosion time. Haematoxylin and eosin staining indicated that apolysis of the integument and wing pad cuticle in the dsLmHR39-treated insects was delayed compared to that in the dsRNA for green fluorescent protein-injected control. Furthermore, RNA-sequencing and RT-qPCR analysis showed the expression level of carboxypeptidase genes (Carboxypeptidase A (CPA) and Carboxypeptidase M (CPM)) and chitin degrading genes (LmChitinase5 (LmCHT5) and LmChitinase10 (LmCHT10)) dramatically declined in the dsLmHR39-treated insects, implying that the LmHR39-mediated 20E signalling pathway is involved in the regulation of carboxypeptidase genes (CPA and CPM) and chitinase genes (LmCHT5 and LmCHT10), and participated in apolysis of the integument and wing pads during locust moulting.
- 相关文献
作者其他论文 更多>>
-
The effects of gamma-aminobutyric acid on growth performance, diarrhoea, ruminal fermentation, and antioxidant capacity in pre-weaned calves
作者：Zhang, Y. T.;Yang, Y.;Bu, D. P.;Ma, L.;Bu, D. P.;Bu, D. P.
关键词：Additives; Antioxidative defence mechanisms; Calf; Faecal score; Rumen development
-
Development and validation of a liquid chromatography-mass spectrometry method for free monosaccharide analysis in milk from 8 species
作者：Qi, Y. J.;Lu, S. C.;Zhao, X. W.;Chen, M. Q.;Song, L.;Zhang, J.;Zheng, N.;Wang, J. Q.;Zhang, Y. D.;Qi, Y. J.;Lu, S. C.;Zhao, X. W.;Chen, M. Q.;Song, L.;Zhang, J.;Zheng, N.;Wang, J. Q.;Zhang, Y. D.;Qi, Y. J.;Lu, S. C.;Zhao, X. W.;Chen, M. Q.;Song, L.;Zhang, J.;Zheng, N.;Wang, J. Q.;Zhang, Y. D.
关键词：free monosaccharide; milk composition; LC-MS; method validation; neonatal nutrition
-
Simultaneous quantification of oxyclozanide and levamisole in milk via ultra-high-performance liquid chromatography tandem mass spectrometry: A validated high-sensitivity approach for veterinary residue surveillance
作者：Dai, G.;Sun, K.;Zhou, Y.;Li, B.;Wang, Q.;Qiu, Y.;Zhou, X.;Zhang, J.;Dai, G.;Sun, K.;Zhang, J.;Peng, X.
关键词：veterinary drug residues; UPLC-MS/MS; food safety; antiparasitic agents; milk
-
Dynamics of oxidative stress and immune responses in neonatal calves during diarrhea
作者：Fu, Z. L.;Yang, Y.;Ma, L.;Bu, D. P.;Fu, Z. L.;Guan, L. L.;Yang, Y.;Malmuthuge, N.;Guan, L. L.
关键词：oxidative stress; calf diarrhea; immune response
-
Inhibition of Aspergillus flavus growth and aflatoxin B-1 production by natamycin
作者：Chang, P.;Zheng, M.;Yang, Q.;Xing, F.;Tai, B.;Xing, F.
关键词：Aspergillus flavus; aflatoxin; natamycin; RNA-seq; SEM; HPLC
-
Polymorphism and Comparative Expression Analysis of THRSP Gene in Fat-Tailed and Thin-Tailed Sheep Breeds
作者：Sun, Q.;Liu, Q.;Di, R.;Wang, Y.;Wang, X.;Hu, W.;Cao, X.;Pan, Zh;Guo, X.;Chu, M.;Gan, S.;Liu, S.;Yang, Y.;Rushdi, H. E.
关键词：Altay sheep; Fat tail; THRSP gene; SNP
-
Dynamic infection ofVerticillium dahliaein upland cotton
作者：Zhang, J.;Hu, H. -L.;Wang, X. -N.;Yang, Y. -H.;Tang, C. -M.;Zhang, C. -J.;Zhu, H. -Q.;Shi, L.;Zhao, M. -W.
关键词：immunity response; infection mechanism; pathogenicity; resistance; upland cotton; Verticillium dahliae
