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Artificial Esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids

文献类型： 外文期刊

作者： Cao Jing 2 ; Wang Miao 1 ; Chen Weihua 3 ; She Yongxin 1 ; Wang Jing 1 ; Wang Fengzhong 3 ; Lao Shuibing 4 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Qual Standardizat & Testing Technol Agroprod, Beijing 100193, Peoples R China

2.Minist Agr, Key Lab Agrofood Safety & Qual Beijing, Beijing 100193, Peoples R China

3.Chinese Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Beijing 100193, Peoples R China

4.Guangxi Acad Agr Sci, Agroprod Qual Safety & Testing Technol Res Inst, Nanning 53003, Peoples R China

关键词： Artificial enzyme; Esterase; Chitosan

期刊名称：CHEMICAL RESEARCH IN CHINESE UNIVERSITIES （ 影响因子：1.307； 五年影响因子：0.979 ）

ISSN： 1005-9040

年卷期： 2019 年 35 卷 3 期

页码：

收录情况： SCI

摘要： A novel artificial esterase based on chitosan and amino acids was synthesized in the present study. The Fmoc-His and Glu were linked to chitosan by active ester method(AEM). The hydroxide radical in chitosan, imidazole group of Fmoc-His and carboxyl from Glu formed a catalytic center of natural esterase. Gel microspheres were coated with a protective layer and a supporting layer by self-assembly construction function in carboxymethylcellulose sodium(CMCS) solution. As for catalytic activity, chitosan-His-Glu was found to be more efficient than chitosan-His and chitosan-Glu in mimicking the core catalytic sites of natural esterase, and the best ratio(mass ratio) of chitosan-His-Glu:CMCS was 1:3. Furthermore, metal ions, such as Ca2+, Mg2+, Fe2+, etc., were able to improve the catalytic efficiency of artificial esterase. And the Lineweaver-Burk plot indicated that the catalytic kinetics of artificial esterase conformed to Michaelis-Menten equation.

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