文献类型： 外文期刊
作者： Lin, Tao 1 ; Chen, Xinglian 1 ; Du, Lijuan 1 ; Wang, Jing 2 ; Hu, Zhengxu 2 ; Cheng, Long 3 ; Liu, Zhenhuan 1 ; Liu, Hongcheng 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Kunming 650205, Peoples R China
2.Longling Agr Environm Protect Monitoring Stn, Baoshan 678300, Peoples R China
3.SCIEX Analyt Instrument Trading Co Ltd, Shanghai 200335, Peoples R China
关键词： time-of-flight mass spectrometry; identification; Dendrobium devonianum; Longling area
期刊名称：FOODS （ 影响因子：5.2； 五年影响因子：5.5 ）
ISSN：
年卷期： 2023 年 12 卷 19 期
页码：
收录情况： SCI
摘要： SWATHtoMRM technology was used in this experiment to further identify and trace the sources of Dendrobium devonianum and Dendrobium officinale produced in the same area using TOF and MS-MRM. After the conversion of the R package of SWATHtoMRM, 191 MRM pairs of positive ions and 96 pairs of negative ions were obtained. Dendrobium devonianum and Dendrobium officinale can be separated very well using the PCA and PLS-DA analysis of MRM ion pairs; this shows that there are obvious differences in chemical composition between Dendrobium devonianum and Dendrobium officinale, which clearly proves that the pseudotargeted metabolomics method based on SWATHtoMRM can be used for traceability identification research. A total of 146 characteristic compounds were obtained, with 20 characteristic compounds in Dendrobium devonianum. The enrichment pathways of the characteristic compounds were mainly concentrated in lipids and atherosclerosis, chagas disease, fluid shear stress and atherosclerosis, proteoglycans in cancer, the IL-17 signaling pathway, the sphingolipid signaling pathway, diabetic cardiomyopathy, arginine and proline metabolism, etc., among which the lipid and atherosclerosis pathways were more enriched, and 11 characteristic compounds affected the expression levels of IL-1, TNF alpha, CD36, IL-1 beta, etc. These can be used as a reference for research on variety improvement and active substance accumulation in Dendrobium devonianum and Dendrobium officinale.
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