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Identification and validation of single-nucleotide polymorphism markers linked to first flower node in kenaf by using combined specific-locus amplified fragment sequencing and bulked segregant analysis

文献类型： 外文期刊

作者： Li Hui 1 ; Li Defang 2 ; Zhao Lining 2 ;

作者机构： 1.Hunan Univ Arts & Sci, Coll Life & Environm Sci, 3150 Dongting Ave, Changde, Hunan, Peoples R China

2.Chinese Acad Agr Sci, Inst Bast Fiber Crops, 348 West XianJiahu Rd, Changsha, Hunan, Peoples R China

关键词： Kenaf; SLAF; BSA

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.645； 五年影响因子：5.749 ）

ISSN： 0926-6690

年卷期： 2019 年 128 卷

页码：

收录情况： SCI

摘要： It is important to develop DNA markers closely linked to the first flower node for molecular marker-assisted selection in kenaf breeding. Kenaf (Hibiscus cannabinus L.) is an annual, multipurpose industrial crop with a worldwide distribution. The higher the node of the first flower, the greater is the fiber yield. In this study, a population of 130 F-2 individuals was constructed through the cross of K215(female) x K89(male). Twenty-five individuals with higher first flower node and twenty-five individuals with lower first flower node were chosen and their DNA were pooled to construct two bulked DNA pools according to the phenotype. Specific-locus amplified fragment sequencing (SLAF-seq) combined with bulked segregant analysis (BSA) was used to identify candidate DNA markers closely linked to the first flower node. Sixteen single-nucleotide polymorphism (SNP) loci were obtained from 11 related SLAF markers associated with the first flower node. SNP locus validation was performed using Sanger sequencing method. The SNP locus S961-2 in Sanger sequencing by using the primer M41961 was consistent with the SNP locus in the related SLAF-seq. The accuracy rate of the genotypes consistent with the first flower node was 91.2% (31/34). To our knowledge, S961-2 is the first SNP locus to be identified that is closely linked to the first flower node. This SNP locus may be useful for marker-assisted selection in breeding of high fiber yielding varieties of kenaf.

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