文献类型： 外文期刊
作者： He Lei 1 ; Chen Guang 1 ; Zhang Sen 1 ; Qiu Zhennan 1 ; Hu Jiang 1 ; Zeng Dali 1 ; Zhang Guangheng 1 ; Dong Guojun 1 ; Gao Z 1 ;
作者机构： 1.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China
2.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejia
关键词： rice; chloroplast transit peptide; translocated function; subcellular location
期刊名称：RICE SCIENCE （ 影响因子：3.333； 五年影响因子：4.226 ）
ISSN： 1672-6308
年卷期： 2019 年 26 卷 1 期
页码：
收录情况： SCI
摘要： Chloroplast transit peptides (CTPs) can be used to transport non-chloroplastic proteins into the chloroplasts. Here, we studied the CTPs of three rice (Oryza sativa L.) chloroplast-localized proteins and found that their CTPs could be used to transport non-chloroplast-localized proteins into the chloroplasts. Fusion proteins lacking the CTP remained located in the cytoplasm. Furthermore, we constructed green fluorescent protein fusion vectors with the three CTPs and three non-chloroplast-localized proteins, Ghd10, MULTI-FLORET SPIKELET1 (MFS1), and SHORTENED UPPERMOST INTERNODE 1 (SUI1). After transforming these constructs into rice protoplasts, the fusion proteins all localized in the chloroplasts. Collectively, our results showed that these CTPs can transport non-chloroplast-localized proteins into the chloroplasts, and more importantly, these CTPs can be applied to engineer chloroplast metabolism.
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