您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Rapid and convenient transformation of cotton (Gossypium hirsutum L.) using in planta shoot apex via glyphosate selection

文献类型： 外文期刊

作者： Guo Wen-Fang 1 ; Wang, Kevin Yueju 2 ; Wang Nan 1 ; Li Jun 1 ; Li Gang-Qiang 1 ; Liu De-Hu 1 ;

作者机构： 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

2.Northeastern State Univ, Dept Nat Sci, Broken Arrow, OK 74014 USA

关键词： shoot apex transformation; CP4-EPSPS; glyphosate; in planta; transgenic cotton

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 10 期

页码：

收录情况： SCI

摘要： Cotton plants are recalcitrant with regards to transformation and induced regeneration. In the present study, 5-enolpyruvylshikimate-3-phosphate (EPSPS), a glyphosate resistant gene from the bacterium Agrobacterium sp. strain CP4, was introduced into an elite Bt transgenic cotton cultivar with a modified technique involving in planta Agrobacteriummediated transformation of shoot apex. Primary transformants were initially screened using a 0.26% glyphosate spray and subsequently by PCR analysis. Five out of 4000 transformants from T-1 seeds were obtained resulting in an in planta transformation rate of 0.125%. Four homozygous lines were produced by continuous self-fertilization and both PCR-based selection and glyphosate resistance. Transgene insertion was analyzed by Southern blot analysis. Gene transcription and protein expression levels in the transgenic cotton lines were further investigated by RT-PCR, Western blot, and ELISA methods. Transgenic T-3 plants were resistant to as much as 0.4% of glyphosate treatments in field trials. Our results indicate that the cotton shoot apex transformation technique which is both tissue-culture and genotype-independent would enable the exploitation of transgene technology in different cotton cultivars. Since this method does not require sterile conditions, the use of specialized growth media or the application of plant hormones, it can be conducted under the greenhouse condition.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台