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Transcriptome, cytological and biochemical analysis of cytoplasmic male sterility and maintainer line in CMS-D8 cotton

文献类型： 外文期刊

作者： Yang, Li 1 ; Wu, Yuanlong 1 ; Zhang, Meng 1 ; Zhang, Jinfa 1 ; Stewart, James McD 1 ; Xing, Chaozhu 1 ; Wu, Jianyong 1 ; Jin, Shuangxia 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

2.Chinese Acad Agr Sci, State Key Lab Cotton Biol, Chinese Acad Agr Sci, Anyang 455000, Henan, Peoples R China

3.New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA

4.Univ Arkansas, Fayetteville, AR 72701 USA

关键词： Cotton; Cytoplasmic male sterility; RNA-Seq; ROS homeostasis; Tapetum development

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN： 0167-4412

年卷期： 2018 年 97 卷 6 期

页码：

收录情况： SCI

摘要： Key message This research based on RNA-seq, biochemical, and cytological analyses sheds that ROS may serve as important signaling molecules of cytoplasmic male sterility in CMS-D8 cotton. To understand the mechanism of cytoplasmic male sterility in cotton (Gossypium hirsutum), transcriptomic, cytological, and biochemical analysis were performed between the cytoplasmic male sterility CMS-D8 line, Zhong41A, and its maintainer line Zhong41B. A total of 2335 differentially expressed genes (DEGs) were identified in the CMS line at three different stages of anther development. Bioinformatics analysis of these DEGs indicated their relationship to reactive oxygen species (ROS) homeostasis, including reduction-oxidation reactions and the metabolism of glutathione and ascorbate. At the same time, DEGs associated with tapetum development, especially the transition to secretory tapetum, were down-regulated in the CMS line. Biochemical analysis indicated that the ability of the CMS line to eliminate ROS was decreased, which led to the rapid release of H2O2. Cytological analysis revealed that the most crucial defect in the CMS line was the abnormal tapetum. All these results are consistent with the RNA sequencing data. On the basis of our findings, we propose that ROS act as signal molecules, which are released from mitochondria and transferred to the nucleus, triggering the formation of abnormal tapetum.

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