Analysis of Choline, Carnitine, Acetylcarnitine and Acetylcholine in Animal Feeds, Blood and Urine Using Ion Chromatography Coupled with Tandem Mass Spectrometry
文献类型： 外文期刊
作者： Suo Decheng 1 ; Fan Xia 1 ; Wei Shulin 1 ; Li Yang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agr Prod, 12 Zhongguancun South St, Beijing 100081, Peoples R China
期刊名称：JOURNAL OF CHROMATOGRAPHIC SCIENCE （ 影响因子：1.28； 五年影响因子：1.28 ）
ISSN： 0021-9665
年卷期： 2021 年 59 卷 5 期
页码：
收录情况： SCI
摘要： A simple and sensitive method was proposed for choline, carnitine, acetylcarnitine (ACa) and acetylcholine (ACh) separation in feed, blood and urine of animals by using ion chromatography (IC) and detection by tandem mass spectrometry (MS/MS). Analytes were extracted using a mixture of acetonitrile and water, purified by C-18 solid-phase extraction columns, separated via IC with an lonPac SCS-1 column and detected by an MS/MS detector by using isotopic internal standards for quantification. The effects of different chromatographic parameters on the separation were also investigated. Under optimal conditions, the recovery was >90%, with the relative standard deviations of <15%. The proposed method was highly reliable for the simultaneous determination of choline, carnitine, ACa and ACh in feed, blood and urine.
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