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Genome-wide identification of the m6A gene family and analysis of m6A methylome in alfalfa under drought stress

文献类型： 外文期刊

作者： Meng, Xiang 1 ; Yi, Dengxia 1 ; Ma, Lin 1 ; Ma, Xiaoran 1 ; Xie, Kaiyun 2 ; Wang, Xuemin 1 ; Yang, Junbo 3 ; Tang, Jun 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China

2.Xinjiang Agr Univ, Coll Grassland Sci, Urumqi 830052, Peoples R China

3.Yazhouwan Natl Lab, Sanya 572024, Peoples R China

关键词： m6A; MeRIP-seq; Abiotic stress; Alfalfa; ABA

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2025 年 228 卷

页码：

收录情况： SCI

摘要： Crops frequently endure diverse environmental stresses that negatively impact their growth and yield. Understanding the mechanisms underlying stress responses is crucial for developing stress-tolerant crop varieties, thereby improving their adaptability and productivity. N6-methyladenosine (m6A) is the most prevalent and reversible internal modification found in eukaryotic mRNA, and is installed, removed, and recognized by methyltransferases (writers), demethylases (erasers), and m6A-binding proteins (readers), playing crucial roles in regulating plant growth, development, and environmental stress response. However, the role of m6A in alfalfa remains largely unexplored. In this work, we conducted comprehensive whole genome identification and expression analysis of the m6A gene family in alfalfa under abiotic stress conditions. There are 11 writers, 6 erasers, and 9 readers were identified in the alfalfa genome. Through comparative genomic analysis, we uncovered the evolutionary relationships of the m6A gene family across different species. Additionally, we characterized the gene structures, conserved motifs, cis-regulatory elements, chromosomal localization, and expression patterns specific to tissues and under various abiotic stress treatments. Furthermore, we conducted a transcriptome-wide analysis of m6A methylome under drought stress. We identified 10,855 hyper-methylated peaks in shoots and 3,262 in roots, along with a global increase in m6A levels during drought stress, individually highlighting the significant role of m6A modification in drought stress responses. Notably, we found that ABA signaling-related genes, such as ABA2, SnRK2.1, SnRK2.3, and SnRK2.4, exhibited m6A hypermethylation in shoots after drought stress treatment. Furthermore, we demonstrated that m6A modifications of ABA2, SnRK2.1, SnRK2.3, and SnRK2.4 enhance their mRNA stability, suggesting that m6A modification may regulate drought stress responses through the ABA signaling pathway. Above all, these findings provide new insights into the adaptive mechanism of alfalfa under drought stress and highlight the potential of regulating m6A modifications as a novel approach for alfalfa stress resistance breeding strategies.

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