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Purification and identification of antioxidant and angiotensin converting enzyme-inhibitory peptides from Guangdong glutinous rice wine

文献类型： 外文期刊

作者： Guo, Junbin 1 ; Lu, Aichun 1 ; Sun, Yunnan 2 ; Liu, Benying 2 ; Zhang, Jinglin 3 ; Zhang, Lingyu 1 ; Huang, Pantian 1 ; Yang, Anping 4 ; Li, Zhenwei 5 ; Cao, Yong 1 ; Miao, Jianyin 1 ;

作者机构： 1.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Nutraceut & Funct Foods, Guangzhou 510642, Guangdong, Peoples R China

2.Yunnan Acad Agr Sci, Tea Res Inst, Yunnan Prov Key Lab Tea Sci, Menghai 666201, Peoples R China

3.Key Lab Brewing Mol Engn China Light Ind, Beijing 100048, Peoples R China

4.Foshan Univ, Sch Med, Foshan 528000, Guangdong, Peoples R China

5.Guangdong Heyuan Luchun Food Co Ltd, Heyuan 517536, Peoples R China

6.Guangxi Normal Univ, State Key Lab Chem & Mol Engn Med Resources, Guilin 541004, Peoples R China

关键词： Glutinous rice wine; Peptides Antioxidant activity; ACE inhibitory Activity; Molecular docking

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.056； 五年影响因子：6.295 ）

ISSN： 0023-6438

年卷期： 2022 年 169 卷

页码：

收录情况： SCI

摘要： Guangdong glutinous rice wine, a type of Chinese yellow rice wine, is rich in a variety of bioactive peptides. In this study, polypeptides with antioxidant and angiotensin converting enzyme (ACE)-inhibitory activities were isolated from Guangdong glutinous rice wine, and their activities and molecular mechanisms were studied. The peptide fraction (F2-2), which exhibited the strongest antioxidant and ACE inhibitory activities, was separated by ultrafiltration and RP-HPLC. A total of 76 peptides were identified by LC-MS/MS, and four peptides with potential antioxidant activities were obtained: VLSGA (445.2536 Da), VISGA (445.2536 Da), MGKAA (476.2417 Da) and GHVAA (453.2336 Da). These four peptides showed significant antioxidant activity with EC50 values of 0.118 mg/mL, 0.056 mg/mL, 0.054 mg/mL and 0.027 mg/mL respectively, and effectively protected cells against oxidative damage. Molecular docking showed that all these four peptides could interact with key active sites on the receptor proteins CD38 and Keap1. Furthermore, these peptides showed good safety and stability according to in silico prediction.

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