Tracking of single virus: Dual fluorescent labeling of pseudorabies virus for observing entry and replication in the N2a cells
文献类型： 外文期刊
作者： Li, Mingzhi 1 ; Pan, Li 1 ; Ma, Caoyuan 1 ; Wu, Hongxia 1 ; Xiang, Guangtao 1 ; Li, Lian-Feng 1 ; Wang, Tao 1 ; Luo, Rui 1 ; Li, Yongfeng 1 ; Liu, Di 1 ; Zhai, Huanjie 1 ; Assad, Moon 1 ; Song, Xin 1 ; Wang, Yanjin 1 ; Gallardo, Franck 2 ; Qiu, Hua-Ji 1 ; Sun, Yuan 1 ;
作者机构： 1.CAAS, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, 678 Haping Rd, Harbin 150069, Heilongjiang, Peoples R China
2.NeoVirTech SAS, Toulouse, France
关键词： ANCHOR DNA labeling system; Envelope; Genome; Live-cell imaging; Pseudorabies virus
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）
ISSN： 0378-1135
年卷期： 2025 年 304 卷
页码：
收录情况： SCI
摘要： Pseudorabies virus (PRV) is a neurotropic herpesvirus. It is not easy to be track the whole replication progress of PRV, especially the nascent viral genome in the host cells. In this study, we developed a dual-fluorescence-labeled PRV (rPRV-Anchor3-mCherry) with the viral genome and the envelope protein gM labeled by ANCHOR DNA labeling system and mCherry, respectively. Through single-virus tracking of rPRV-Anchor3-mCherry, we observed that PRV invaded mouse neuroblastoma Neuro-2a cells via both endocytosis and plasma membrane fusion pathway. During the replication stage, parental and progeny viral genome of rPRV-Anchor3-mCherry in the cell nuclei could be visible, and viral nucleocapsid appeared more specifically than traditional capsid protein labeled PRV particles (rPRV-VP26-EGFP). We found that numerous progeny viral particles were produced in the nuclear, causing the nucleus membrane to break using three-dimensional (3D) live-cell imaging and electron microscopy. Moreover, our findings confirmed that simultaneously targeting of the UL9 and UL54 genes using a CRISPR-Cas9 system led to the complete inhibition PRV replication. rPRV-Anchor3-mCherry can be used to research multiple steps of the viral cycle.
- 相关文献
作者其他论文 更多>>
-
Molecular mechanism of potently neutralizing human monoclonal antibodies against severe fever with thrombocytopenia virus infection
作者：Quan, Chuansong;Nie, Kaixiao;Zheng, Wenjun;Wang, Yiwen;Quan, Chuansong;Zhang, Hong;Quan, Chuansong;Ma, Dezhen;Su, Chao;Li, Lianfeng;Peng, Dingkun;Yin, Chunhong;Yang, Peipei;Shan, Chao;Liu, Xin;Zheng, Jie;Shi, Weifeng;Li, Weiwei;Gao, George F.;Qi, Jianxun;Liu, Weixiao;Liu, Di;Carr, Michael J.;Carr, Michael J.;Shi, Weifeng
关键词：severe fever with thrombocytopenia syndrome; SFTSV; Bandavirus dabieense; monoclonal antibodies; neutralization; therapeutics
-
The S273R protein of African swine fever virus antagonizes the canonical NF-κB signaling pathway by IκBα
作者：Ren, Haojie;Shi, Lan-Fang;Pan, Xiao-Ya;Ma, Yu-He;Fan, Jun-Hao;Chen, Xing;Yang, Zhong-Yuan;Fan, Shuai;Zhang, Yuhang;Han, Shichong;He, Wen-Rui;Wan, Bo;Zhang, Gai-Ping;Wang, Yanjin;Li, Su;Qiu, Hua-Ji;Zhang, Yuhang;Han, Shichong;He, Wen-Rui;Wan, Bo;Zhang, Gai-Ping;Zhang, Yuhang;Han, Shichong;He, Wen-Rui;Wan, Bo
关键词：African swine fever virus; canonical NF-
kappa B signaling pathway; S273R protein; Ikappa Balpha ; inflammatory responses -
COP I vesicles facilitate classical swine fever virus proliferation by transporting fatty acid synthase from the Golgi apparatus to the endoplasmic reticulum
作者：Zhang, Liang;Chen, Chen;Song, Mengzhao;Li, Ning;Luo, Bihao;Quan, Yuehan;Guo, Kangkang;Zhang, Yanming;Wang, Tao
关键词：classical swine fever virus; early secretory pathway; coatomer protein I; coatomer protein II; fatty acid synthase
-
Two telomere-to-telomere pig genome assemblies and pan-genome analyses provide insights into genomic structural landscape and genetic adaptations
作者：Zong, Wencheng;Chen, Li;Wang, Jinbu;Hou, Xinhua;Liu, Xin;Wang, Ligang;Wang, Lixian;Zhang, Longchao;Chen, Li;Chai, Jie;Wang, Jinyong;Zhang, Dongjie;Tian, Ming;He, Xinmiao;Liu, Di;Zhang, Yuebo;He, Jun;Yin, Yulong;Zhang, Yuebo;He, Jun;Yin, Yulong;An, Yalong;Song, Chengyi;D'Alessandro, Enrico;Li, Mingzhou
关键词：
-
The molecular adjuvant effect of the C-terminal peptide of complement C5a in a teleost fish
作者：Liu, Bin;Liang, Jia-Xin;Ma, Zi-You;Zhong, Ya-Qin;Sun, Yuan;Zhang, Yong-An;Zhang, Xu-Jie;Wang, Xin-Yuan;Zhang, Yong-An;Zhang, Xu-Jie;Zhang, Yong-An;Zhang, Xu-Jie;Zhang, Yong-An;Zhang, Xu-Jie;Zhang, De-Feng
关键词：C5a; Chemotactic activity; Phagocytic activity; Molecular adjuvant; Grass carp
-
Widely Targeted Metabolomic and Network Pharmacology Analyses of Active Compounds Enriched from Ethanolic Extract of Oudemansiella raphanipes
作者：Wu, Zhi;Zhao, Jin;Lin, Junbin;Miao, Renyun;Feng, Rencai;Gan, Bingcheng;Wang, Tao;Zhu, Shuang;Chen, Mengxing;Wu, Dan;Wu, Yiyou;Li, Xiang;Gan, Bingcheng
关键词：
Oudemansiella raphanipes ; widely targeted metabolomics; antioxidant activity; enrichment approaches; network pharmacology -
Platycodon grandiflorum-derived extracellular vesicles suppress triple-negative breast cancer growth by reversing the immunosuppressive tumor microenvironment and modulating the gut microbiota
作者：Yang, Min;Guo, Jia;Li, Jinxian;Sun, Yuan;Liu, Ying;Peng, Yinghua;Wang, Shuyue
关键词：
Platycodon grandiflorum ; Plant-derived extracellular vesicles; TNBC; Tumor immunotherapy; Gut microbiota modulation
